Dube Yvonne, Khan Amber, Marimani Musa, Ahmad Aijaz
Department of Clinical Microbiology and Infectious Diseases, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, South Africa.
Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, South Africa.
Can J Microbiol. 2020 Dec;66(12):733-747. doi: 10.1139/cjm-2019-0491. Epub 2020 Aug 10.
Candidiasis caused by multidrug-resistant species continues to be difficult to eradicate. The use of live probiotic bacteria has gained a lot of interest in the treatment of candidiasis; however, whole-cell probiotic use can often be associated with a high risk of sepsis. Strategies manipulating cell-free methods using probiotic strains could lead to the development of novel antifungal solutions. Therefore, we evaluated the effect of three probiotic cell-free extracts (CFEs) on the growth, virulence traits, and drug efflux pumps in On the basis of its minimum inhibitory concentration, was selected and assessed against various virulence traits and drug resistance mechanisms. The results showed that CFE significantly inhibited hyphae formation and reduced secretion of proteinases and phospholipases. Moreover, inhibited the drug efflux proteins in resistant strains thus reversing drug resistance. Gene expression data confirmed downregulation of genes associated with microbial virulence and drug resistance following treatment of with CFE. Through gas chromatography - mass spectrometry chemical characterization, high contents of oleic acid (24.82%) and myristic acid (13.11%) were observed in this CFE. Collectively, our findings indicate that may potentially be used for therapeutic purposes to inhibit infections.
由多重耐药菌种引起的念珠菌病仍然难以根除。使用活的益生菌在念珠菌病治疗中引起了广泛关注；然而，使用全细胞益生菌通常与败血症的高风险相关。利用益生菌菌株采用无细胞方法的策略可能会促成新型抗真菌解决方案的开发。因此，我们评估了三种益生菌无细胞提取物（CFE）对白色念珠菌生长、毒力特性和药物外排泵的影响。基于其最低抑菌浓度，选择了[具体菌株]并针对各种毒力特性和耐药机制进行评估。结果表明，[具体CFE]显著抑制菌丝形成，并减少蛋白酶和磷脂酶的分泌。此外，[具体CFE]抑制耐药白色念珠菌菌株中的药物外排蛋白，从而逆转耐药性。基因表达数据证实，用[具体CFE]处理白色念珠菌后，与微生物毒力和耐药性相关的基因表达下调。通过气相色谱 - 质谱化学表征，在该CFE中观察到高含量的油酸（24.82%）和肉豆蔻酸（13.11%）。总体而言，我们的研究结果表明，[具体CFE]可能具有治疗用途，可用于抑制白色念珠菌感染。
