Song Young-Gyun, Lee Sung-Hoon
Department of Prosthodontics, College of Dentistry, Dankook University, Cheonan, Republic of Korea.
Department of Oral Microbiology and Immunology, College of Dentistry, Republic of Korea.
Arch Oral Biol. 2017 Apr;76:1-6. doi: 10.1016/j.archoralbio.2016.12.014. Epub 2016 Dec 31.
Candida albicans biofilm is associated with denture-related stomatitis and oral candidiasis of elderly. Probiotics are beneficial bacteria and have antibacterial activity against pathogenic bacteria. The purpose of this study was to investigate the antifungal activity of various probiotics against C. albicans and the inhibitory effects of probiotics on Candida biofilm on the denture surface.
The spent culture media of various probiotics were investigated the antifungal efficacy against C. albicans. Candida biofilm was formed on a denture base resin and was then treated with Lactobacillus rhamnosus and Lactobacillus casei. Also, the biofilms of L. rhamnosus and L. casei were formed and were sequentially treated with C. albicans. Colony-forming units of C. albicans on the denture surface were counted after spreading on agar plate. The denture base resin was treated with the spent culture media for 30days, after which the denture surface roughness was analyzed with an atomic force microscope.
L. rhamnosus and L. casei exhibited stronger antifungal activity than other probiotics. The spent culture medium of L. rhamnosus and L. casei exhibited the antifungal activity against blastoconidia and biofilm of C. albicans. L. rhamnosus and L. casei showed the antifungal activity against Candida biofilm, and the biofilm of L. rhamnosus and L. casei inhibited formation of Candida biofilm on denture surface. Neither of the probiotics affected the surface roughness of the denture base resin.
L. rhamnosus and L. casei may be the ideal probiotics for the prevention and treatment of denture-related stomatitis.
白色念珠菌生物膜与老年义齿性口炎及口腔念珠菌病相关。益生菌是有益菌，对病原菌具有抗菌活性。本研究旨在探讨各种益生菌对白色念珠菌的抗真菌活性以及益生菌对义齿表面念珠菌生物膜的抑制作用。
研究各种益生菌的用过的培养基对白色念珠菌的抗真菌效果。在义齿基托树脂上形成念珠菌生物膜，然后用鼠李糖乳杆菌和干酪乳杆菌处理。此外，形成鼠李糖乳杆菌和干酪乳杆菌的生物膜，并依次用白色念珠菌处理。将义齿表面的白色念珠菌菌落形成单位铺在琼脂平板上后进行计数。用用过的培养基处理义齿基托树脂30天，之后用原子力显微镜分析义齿表面粗糙度。
鼠李糖乳杆菌和干酪乳杆菌表现出比其他益生菌更强的抗真菌活性。鼠李糖乳杆菌和干酪乳杆菌的用过的培养基对白色念珠菌的芽生孢子和生物膜表现出抗真菌活性。鼠李糖乳杆菌和干酪乳杆菌对念珠菌生物膜表现出抗真菌活性，并且鼠李糖乳杆菌和干酪乳杆菌的生物膜抑制义齿表面念珠菌生物膜的形成。两种益生菌均未影响义齿基托树脂的表面粗糙度。
鼠李糖乳杆菌和干酪乳杆菌可能是预防和治疗义齿性口炎的理想益生菌。
