Suppr超能文献

硒代蛋氨酸对 T-2 毒素诱导的肠道损伤的保护作用。

Protective effect of selenomethionine on intestinal injury induced by T- 2 toxin.

机构信息

College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan 471000, China.

College of life science, Yangtze University, Jingzhou, Hubei 434023, China.

出版信息

Res Vet Sci. 2020 Oct;132:439-447. doi: 10.1016/j.rvsc.2020.07.018. Epub 2020 Aug 1.

Abstract

T-2 toxin is the most toxic as a type A trichothecenes, which could contaminate grains, especially in wheat and corn. It can cause immune suppression, neurotoxicity, the apoptosis of cells and even induce tumorigenesis. Recent studies have indicated that selenium (Se) have protective effect against mycotoxins-induced toxicity. The present studies was designed to investigate the protective role of Selenomethionine (SeMet) on T-2 toxin-induced toxicity in rabbit's jejunum. 50 New Zealand rabbits were divided into five group (Control group, T-2 group, low-dose Se + T-2 group, medium-dose + T-2 group and high-dose Se + T-2 group). New Zealand rabbits were orally administered with SeMet (0.2, 0.4 and 0.6 mg/kg, Adding diet) for 21 days. On 17th days, each group began to take 0.4 mg/kg of T-2 toxin orally every day for 5 days. We found that rabbit exposed to T-2 toxin could increase the levels of ROS, and decrease activities of antioxidant enzymes and the expression of Occludin and ZO-1. In addition, T-2 toxin could trigger jejunal inflammatory response and enhance the expression of IL-1β, IL-6 and TNF-α. After SeMet pretreatment, our results indicated that Se attenuated the T-2 toxin-induced oxidative stress, decreasing the level of ROS, MDA and enhancing the activity of SOD and GSH-Px. Moreover, SeMet can alleviate jejunal inflammatory response, and protect the integrity of the intestinal barrier through up-regulating the expression of ZO-1 and Occludin. In the present research, supplementation of 0.2 mg/kg SeMet in the diet could effectively alleviate the T-2 toxin poisoning in rabbits.

摘要

T-2 毒素是 A 型单端孢霉烯族毒素中毒性最强的一种,可污染谷物,尤其是小麦和玉米。它可以导致免疫抑制、神经毒性、细胞凋亡,甚至诱导肿瘤发生。最近的研究表明,硒(Se)对霉菌毒素诱导的毒性具有保护作用。本研究旨在探讨硒蛋氨酸(SeMet)对 T-2 毒素诱导的兔空肠毒性的保护作用。将 50 只新西兰兔分为五组(对照组、T-2 组、低剂量 Se+T-2 组、中剂量 Se+T-2 组和高剂量 Se+T-2 组)。新西兰兔通过饮食添加 0.2、0.4 和 0.6mg/kg 的 SeMet 连续 21 天。第 17 天,每组开始每天口服 0.4mg/kg 的 T-2 毒素,连续 5 天。我们发现,暴露于 T-2 毒素的兔可增加 ROS 水平,降低抗氧化酶活性以及 Occludin 和 ZO-1 的表达。此外,T-2 毒素可引发空肠炎症反应,增强 IL-1β、IL-6 和 TNF-α的表达。经 SeMet 预处理后,我们的结果表明,Se 可减轻 T-2 毒素诱导的氧化应激,降低 ROS、MDA 水平,增强 SOD 和 GSH-Px 的活性。此外,SeMet 可通过上调 ZO-1 和 Occludin 的表达来减轻空肠炎症反应,保护肠道屏障的完整性。在本研究中,饮食中添加 0.2mg/kg 的 SeMet 可有效缓解兔的 T-2 毒素中毒。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验