Sokolowska Patrycja, Janikiewicz Justyna, Jastrzebska Elzbieta, Brzozka Zbigniew, Dobrzyn Agnieszka
Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Poland; Laboratory of Cell Signaling and Metabolic Disorders, Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, Poland.
Laboratory of Cell Signaling and Metabolic Disorders, Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, Poland.
Biosens Bioelectron. 2020 Nov 1;167:112451. doi: 10.1016/j.bios.2020.112451. Epub 2020 Jul 25.
Cases of type 2 diabetes mellitus have significantly increased in recent years. Researchers worldwide are combining their knowledge of biology, medicine, tissue engineering, and microtechnology to develop new effective treatments. An important aspect of current research is to develop of a complete model of three-dimensional pancreatic islets to test various factors that affect disease development and evaluate new therapies and drugs. Several methods have allowed the development of three-dimensional research models. The use of Lab-on-a-chip systems with appropriate microstructure geometry is a promising solution to macroscale problems. Such a device allows the development of a complete platform reflecting conditions that prevail in the body. Organ-on-a-chip platforms are successfully used mainly in studies of lung, heart, and liver diseases. This review presents the current state of knowledge on the creation of three-dimensional pancreatic islet structures in both microscale and microfluidic systems. We highlight the most important aspects of developing the geometry of such devices. We also discuss analytical detection methods that are suitable for detecting hormones that are secreted from pancreatic islets and, in combination with appropriate Lab-on-a-chip systems, can be used as a Micro Total Analysis System (μTAS).
近年来，2型糖尿病病例显著增加。世界各地的研究人员正在整合他们在生物学、医学、组织工程和微技术方面的知识，以开发新的有效治疗方法。当前研究的一个重要方面是建立一个完整的三维胰岛模型，以测试影响疾病发展的各种因素，并评估新的治疗方法和药物。有几种方法可以开发三维研究模型。使用具有适当微观结构几何形状的芯片实验室系统是解决宏观问题的一个有前途的方案。这样的设备可以开发出一个反映体内实际情况的完整平台。芯片上器官平台主要成功应用于肺部、心脏和肝脏疾病的研究。这篇综述介绍了在微观尺度和微流控系统中创建三维胰岛结构的当前知识状态。我们强调了开发此类设备几何形状的最重要方面。我们还讨论了适用于检测胰岛分泌激素的分析检测方法，这些方法与适当的芯片实验室系统相结合，可作为微全分析系统（μTAS）使用。
