Biosensors for Bioengineering Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Baldiri I Reixac, 10-12, 08028 Barcelona, Spain.
Plasmon Nano-Optics Group, ICFO-Institute for Photonics Sciences, The Barcelona Institute of Science and Technology, 08860 Barcelona, Spain.
Biosensors (Basel). 2021 Apr 28;11(5):138. doi: 10.3390/bios11050138.
Organ-on-a-chip (OOC) devices offer new approaches for metabolic disease modeling and drug discovery by providing biologically relevant models of tissues and organs in vitro with a high degree of control over experimental variables for high-content screening applications. Yet, to fully exploit the potential of these platforms, there is a need to interface them with integrated non-labeled sensing modules, capable of monitoring, in situ, their biochemical response to external stimuli, such as stress or drugs. In order to meet this need, we aim here to develop an integrated technology based on coupling a localized surface plasmon resonance (LSPR) sensing module to an OOC device to monitor the insulin in situ secretion in pancreatic islets, a key physiological event that is usually perturbed in metabolic diseases such as type 2 diabetes (T2D). As a proof of concept, we developed a biomimetic islet-on-a-chip (IOC) device composed of mouse pancreatic islets hosted in a cellulose-based scaffold as a novel approach. The IOC was interfaced with a state-of-the-art on-chip LSPR sensing platform to monitor the in situ insulin secretion. The developed platform offers a powerful tool to enable the in situ response study of microtissues to external stimuli for applications such as a drug-screening platform for human models, bypassing animal testing.
器官芯片（OOC）设备通过提供高度控制实验变量的体外组织和器官的生物学相关模型，为代谢疾病建模和药物发现提供了新方法，适用于高通量筛选应用。然而，为了充分利用这些平台的潜力，需要将它们与集成的非标记传感模块相连接，这些模块能够原位监测其对外部刺激（如应激或药物）的生化反应。为了满足这一需求，我们旨在开发一种基于局部表面等离子体共振（LSPR）传感模块与 OOC 设备相结合的集成技术，以监测胰岛中的胰岛素原位分泌情况，这是一种关键的生理事件，通常在代谢疾病（如 2 型糖尿病（T2D））中受到干扰。作为概念验证，我们开发了一种仿生胰岛芯片（IOC）设备，由作为新型方法的纤维素支架中容纳的小鼠胰岛组成。该 IOC 与最先进的片上 LSPR 传感平台相连接，以原位监测胰岛素的分泌情况。该开发平台为微组织对外部刺激的原位反应研究提供了有力工具，可用于药物筛选平台等人类模型，从而绕过动物试验。
