Shapira Y, Shohami E, Sidi A, Soffer D, Freeman S, Cotev S
Department of Anesthesiology, Hadassah University Hospital, Jerusalem, Israel.
Crit Care Med. 1988 Mar;16(3):258-65. doi: 10.1097/00003246-198803000-00010.
A model of closed head injury in rats was developed using a calibrated weight-drop device. The development of edema was studied in various brain regions (cerebral hemispheres, brain stem, cerebellum) using a linear specific gravity gradient column. Regional brain tissue density was measured within 1 min, at 15 and 60 min, 18 h, 4 and 10 days after injury to the left cerebral hemisphere, and was compared with values in sham-operated and control rats. Significant edema (i.e., reduced specific gravity) occurred only in the traumatized hemisphere and was maximal at 18 h. A neurologic severity score (NSS) was developed to evaluate the status of the rat after injury. Specific gravity was significantly correlated with NSS at 18 h after injury. The affected hemisphere displayed hemorrhagic lesions as early as one hour post head trauma (HT), which evolved into hemorrhagic necrosis at 18 h. A pathologic score, evaluated 18 h post HT based on size and severity of the lesion, was correlated with the NSS and evaluated for each rat at one hour and 18 h postimpact. This correlation was found to be highly significant. This model of brain injury may be useful in future studies on the effects of therapeutic agents.
使用校准的重物下落装置建立大鼠闭合性颅脑损伤模型。使用线性比重梯度柱研究了不同脑区（大脑半球、脑干、小脑）水肿的发展情况。在损伤左大脑半球后的1分钟、15和60分钟、18小时、4天和10天内测量局部脑组织密度，并与假手术组和对照组大鼠的值进行比较。显著水肿（即比重降低）仅发生在受创伤的半球，且在18小时时达到最大。制定了神经严重程度评分（NSS）以评估大鼠受伤后的状态。损伤后18小时时比重与NSS显著相关。受影响的半球在头部创伤（HT）后1小时就出现出血性病变，在18小时时发展为出血性坏死。基于损伤的大小和严重程度在HT后18小时评估的病理评分与NSS相关，并在撞击后1小时和18小时对每只大鼠进行评估。发现这种相关性非常显著。这种脑损伤模型可能对未来治疗药物效果的研究有用。
