Baki Adile Merve, Aydın Abdurrahman Fatih, Vural Pervin, Olgaç Vakur, Doğru Abbasoğlu Semra, Uysal Müjdat
Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.
Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. Electronic Address:
Cell J. 2020 Jul;22(Suppl 1):117-124. doi: 10.22074/cellj.2020.7049. Epub 2020 Jul 18.
There are controversial data about the prooxidant-antioxidant balance in hypothyroidism. We aimed to investigate the effect of α-lipoic acid (ALA) on oxidative stress parameters in the liver and brain of propylthiouracil (PTU)-induced hypothyroid rats.
In this experimental study, PTU (500 mg/L) was given to rats in drinking water for 10 weeks. ALA (0.2% in diet) alone and together with thyroxine (T4, 20 μg/kg body weight, s.c) were given to hypothyroid rats in the last 5 weeks of the experimental period. The levels of reactive oxygen species, malondialdehyde, protein carbonyl, ferric reducing antioxidant power (FRAP) and glutathione (GSH) levels, superoxide dismutase, and GSH peroxidase activities were determined in the liver and brain of rats. Histopathological examinations were also performed.
Prooxidant parameters were increased in the brain but not liver in hypothyroid rats. ALA treatment alone lowered enhanced brain oxidative stress in hypothyroid rats. Also, ALA was found to ameliorate the changes as a result of oxidative stress arising from T4 replacement therapy.
Our results indicate that ALA alone and together with T4 may be useful in reducing oxidative stress in thyroid dysfunctions.
关于甲状腺功能减退症中促氧化剂 - 抗氧化剂平衡存在有争议的数据。我们旨在研究α - 硫辛酸（ALA）对丙硫氧嘧啶（PTU）诱导的甲状腺功能减退大鼠肝脏和大脑氧化应激参数的影响。
在本实验研究中，给大鼠饮用含500 mg/L PTU的水，持续10周。在实验期的最后5周，单独给予甲状腺功能减退大鼠ALA（饮食中含0.2%）以及与甲状腺素（T4，20 μg/kg体重，皮下注射）联合使用。测定大鼠肝脏和大脑中的活性氧、丙二醛、蛋白质羰基、铁还原抗氧化能力（FRAP）和谷胱甘肽（GSH）水平、超氧化物歧化酶以及GSH过氧化物酶活性。还进行了组织病理学检查。
甲状腺功能减退大鼠大脑中的促氧化剂参数增加，但肝脏中未增加。单独使用ALA治疗可降低甲状腺功能减退大鼠大脑中增强的氧化应激。此外，发现ALA可改善因T4替代疗法引起的氧化应激变化。
我们的结果表明，单独使用ALA以及与T4联合使用可能有助于减轻甲状腺功能障碍中的氧化应激。
