Bio Environmental Health Hazards Research Center, Jiroft University of Medical Sciences, Jiroft, Iran.
Student Research Committee, Jiroft University of Medical Sciences, Jiroft, Iran.
J Complement Integr Med. 2024 Jan 22;21(1):123-130. doi: 10.1515/jcim-2023-0147. eCollection 2024 Mar 1.
This study aimed to investigate the antioxidant effect of rosiglitazone (ROG) and pioglitazone (POG) on oxidative damage and dysfunction of hepatic tissue in hypothyroid rats.
The male rats were classified into six groups: (1) Control; (2) Hypothyroid, (3) Hypothyroid-POG 10, (4) Hypothyroid-POG 20, (5) Hypothyroid-ROG 2, and (6) Hypothyroid-ROG 4. To induction hypothyroidism in rats, propylthiouracil (PTU) (0.05 %w/v) was added to drinking water. In groups 2-6, besides PTU, the rats were also intraperitoneal administrated with 10 or 20 mg/kg POG or 2 or 4 mg/kg ROG for six weeks. Finally, after deep anesthesia, the blood was collected to measure the serum biochemical markers and hepatic tissue was separated for biochemical oxidative stress markers.
Administration of PTU significantly reduced serum thyroxin concentration, total thiol levels, activity of superoxide dismutase (SOD) and catalase (CAT) enzymes, and increased serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (Alk-P) and malondialdehyde (MDA) in the liver. Additionally, our results showed that prescription of POG or ROG for six weeks to hypothyroid rats resulted in an improvement in liver dysfunction (decrease in serum levels of AST, ALT, and ALK-P) through reducing oxidative damage in hepatic tissue (increase in CAT, SOD, or total thiols and decrease in MDA levels).
The findings of the present study presented that the IP administration of POG and ROG for six weeks improves liver dysfunction induced by hypothyroidism in juvenile rats by reducing oxidative damage.
本研究旨在探讨罗格列酮（ROG）和吡格列酮（POG）对甲状腺功能减退大鼠肝组织氧化损伤和功能障碍的抗氧化作用。
雄性大鼠分为六组：（1）对照组；（2）甲状腺功能减退组；（3）甲状腺功能减退-吡格列酮 10 组；（4）甲状腺功能减退-吡格列酮 20 组；（5）甲状腺功能减退-罗格列酮 2 组；（6）甲状腺功能减退-罗格列酮 4 组。为诱导大鼠甲状腺功能减退，在饮用水中添加丙硫氧嘧啶（PTU）（0.05%w/v）。在第 2-6 组中，除了 PTU 外，大鼠还腹膜内注射 10 或 20mg/kg POG 或 2 或 4mg/kg ROG，持续 6 周。最后，在深度麻醉后，采集血液以测量血清生化标志物，并分离肝组织以测量生化氧化应激标志物。
PTU 给药显著降低了血清甲状腺素浓度、总巯基水平、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性，同时增加了血清天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（Alk-P）和丙二醛（MDA）在肝脏中的含量。此外，我们的结果表明，将 POG 或 ROG 处方给甲状腺功能减退大鼠六周可通过减少肝组织氧化损伤（CAT、SOD 或总巯基增加和 MDA 水平降低）来改善肝功能障碍（血清 AST、ALT 和 ALK-P 水平降低）。
本研究结果表明，IP 给予 POG 和 ROG 六周可减轻氧化损伤，改善幼年大鼠甲状腺功能减退引起的肝功能障碍。
