Nagashima T, Hoshino T, Cho K G, Senegor M, Waldman F, Nomura K
Brain Tumor Research Center, University of California, San Francisco.
J Neurosurg. 1988 Mar;68(3):388-92. doi: 10.3171/jns.1988.68.3.0388.
Sixteen patients with brain tumors were given a 30- to 60-minute intravenous infusion of bromodeoxyuridine (BUdR), 200 mg/sq m. Grossly viable fragments were taken from the biopsied tumor specimens and divided into two portions. One portion was dissociated into single cells, stained both with fluorescein isothiocyanate (FITC) using anti-BUdR monoclonal antibody as the first antibody and with propidium iodide (for deoxyribonucleic acid), and analyzed by flow cytometry (FCM). The labeling index (LI) was calculated as the number of FITC-labeled cells expressed as a percentage of the total number of cells analyzed. The other portion was fixed in 70% ethanol, embedded in paraffin, sectioned, and stained with immunoperoxidase using anti-BUdR monoclonal antibody as the first antibody. The LI of these tissue sections was calculated in two ways: from selected areas in which the labeled cells were evenly distributed and from the entire tissue section. The LI's obtained by FCM correlated closely with those from the entire tissue sections (r = 0.99, p less than 0.000001) and were usually lower than LI's from selected areas of tissue sections. The LI's determined by FCM also correlated well with the LI's from selected areas of tissue sections (r = 0.82, p less than 0.00012), despite the difference in values between them. Thus, the FCM-derived LI and the tissue LI can both provide useful information for predicting the biological malignancy of individual tumors and for designing treatment regimens for individual patients with brain tumors; however, different standards should be used to interpret the LI's obtained by these two methods.
16例脑肿瘤患者接受了30至60分钟的静脉输注溴脱氧尿苷（BUdR），剂量为200mg/平方米。从活检的肿瘤标本中获取大体上有活力的碎片，并分成两份。一份解离成单细胞，先用抗BUdR单克隆抗体作为一抗用异硫氰酸荧光素（FITC）染色，再用碘化丙啶（用于脱氧核糖核酸）染色，然后通过流式细胞术（FCM）进行分析。标记指数（LI）计算为FITC标记细胞的数量占所分析细胞总数的百分比。另一份固定在70%乙醇中，石蜡包埋，切片，并用抗BUdR单克隆抗体作为一抗进行免疫过氧化物酶染色。这些组织切片的LI通过两种方式计算：从标记细胞均匀分布的选定区域以及从整个组织切片计算。通过FCM获得的LI与整个组织切片的LI密切相关（r = 0.99，p < 0.000001），并且通常低于组织切片选定区域的LI。尽管它们之间的值存在差异，但通过FCM确定的LI与组织切片选定区域的LI也具有良好的相关性（r = 0.82，p < 0.00012）。因此，FCM得出的LI和组织LI都可为预测个体肿瘤的生物学恶性程度以及为个体脑肿瘤患者设计治疗方案提供有用信息；然而，应使用不同的标准来解释通过这两种方法获得的LI。
