Tecnológico Nacional de México/Instituto Tecnológico de Toluca, Av. Tecnológico S/n. Colonia Agrícola Bellavista, Metepec, Estado de México, C.P. 52149, Mexico.
Tecnológico Nacional de México/Instituto Tecnológico de Toluca, Av. Tecnológico S/n. Colonia Agrícola Bellavista, Metepec, Estado de México, C.P. 52149, Mexico.
Anal Biochem. 2020 Nov 1;608:113897. doi: 10.1016/j.ab.2020.113897. Epub 2020 Aug 8.
The azo dyes, Yellow 5 (Y5), Red 2 (R2) and Blue 1 (B1), quantified in solutions and in mixtures of binary dyes, were studied by means of UV-Vis spectroscopy. In this work was used a CIE algorithm developed in Visual Basic for Applications (VBA). The CIE algorithm is based on the tristimulus chromaticity diagram, as an alternative to the shielding effect that arises in dye mixtures, and it can also be applied to complex quantification methods such as HPLC (High Performance Liquid Chromatography). The results obtained through of the algorithm, showed a higher accuracy from 97 to 99% in relation with similar UV-Vis quantification methods. In contrast, linear methods only managed to reach an accuracy from 78 to 98%. Additionally, the algorithm yielded significant similar values to the UHPLC reference method. The results showed that the method CIE algorithm was accessible and reliable to quantify binary mixtures of the dyes used which suggests the possibility to apply this method on other dyes, within the limits of quantification obtained in this study (0.076-24.56 mg/L) and the pH values from 2 to 10.
偶氮染料黄色 5（Y5）、红色 2（R2）和蓝色 1（B1）在溶液中和二元染料混合物中通过紫外可见光谱进行了定量研究。在这项工作中，使用了在 Visual Basic for Applications (VBA) 中开发的 CIE 算法。CIE 算法基于三刺激色度图，是对染料混合物中屏蔽效应的替代方法，也可应用于复杂的定量方法，如高效液相色谱 (HPLC)。通过算法得到的结果，与类似的紫外可见定量方法相比，其准确性从 97%到 99%。相比之下，线性方法只能达到 78%到 98%的准确性。此外，算法得到的结果与 UHPLC 参考方法有显著的相似值。结果表明，CIE 算法方法易于访问且可靠，可用于定量所用染料的二元混合物，这表明该方法有可能在本研究获得的定量限（0.076-24.56mg/L）和 pH 值 2-10 范围内应用于其他染料。
