Tominac Trcin Mirna, Zdraveva Emilija, Dolenec Tamara, Vrgoč Zimić Ivana, Bujić Mihica Marina, Batarilo Ivanka, Dekaris Iva, Blažević Valentina, Slivac Igor, Holjevac Grgurić Tamara, Bajsić Emi Govorčin, Markov Ksenija, Čanak Iva, Kuzmić Sunčica, Tarbuk Anita, Tomljenović Antoneta, Mrkonjić Nikolina, Mijović Budimir
The Institute of Immunology, Rockefellerova ul. 2, 10000 Zagreb, Croatia.
Faculty of Textile Technology, University of Zagreb, Prilaz baruna Filipovića 28a, 10000 Zagreb, Croatia.
Polymers (Basel). 2020 Aug 6;12(8):1758. doi: 10.3390/polym12081758.
Limbal Stem Cell Deficiency (LSCD) is a very serious and painful disease that often results in impaired vision. Cultivation of limbal stem cells for clinical application is usually performed on carriers such as amniotic membrane or surgical fibrin gel. Transplantation of these grafts is associated with the risk of local postoperative infection that can destroy the graft and devoid therapeutic benefit. For this reason, electrospun scaffolds are good alternatives, as proven to mimic the natural cells surroundings, while their fabrication technique is versatile with regard to polymer functionalization and scaffolds architecture. This study considers the development of poly(ε-caprolactone) (PCL) immune-compatible and biodegradable electrospun scaffolds, comprising cefuroxime (CF) or titanium dioxide (TiO) active components, that provide both bactericidal activity against eye infections and support of limbal stem cells growth in vitro. The PCL/CF scaffolds were prepared by blend electrospinning, while functionalization with the TiO particles was performed by ultrasonic post-processing treatment. The fabricated scaffolds were evaluated in regard to their physical structure, wetting ability, static and dynamic mechanical behaviour, antimicrobial efficiency and drug release, through scanning electron microscopy, water contact angle measurement, tensile testing and dynamic mechanical analysis, antimicrobial tests and UV-Vis spectroscopy, respectively. Human limbal stem cells, isolated from surgical remains of human cadaveric cornea, were cultured on the PCL/CF and PCL/TiO scaffolds and further identified through immunocytochemistry in terms of cell type thus were stained against p63 marker for limbal stem cells, a nuclear transcription factor and cytokeratin 3 (CK3), a corneal epithelial differentiation marker. The electrospun PCL/CF and PCL/TiO successfully supported the adhesion, proliferation and differentiation of the cultivated limbal cells and provided the antimicrobial effect against and
角膜缘干细胞缺乏症（LSCD）是一种非常严重且痛苦的疾病，常常导致视力受损。用于临床应用的角膜缘干细胞培养通常在诸如羊膜或外科纤维蛋白凝胶等载体上进行。这些移植物的移植存在局部术后感染的风险，这种感染可能会破坏移植物并失去治疗益处。因此，电纺支架是很好的替代物，因为已证明其能模拟天然细胞环境，而且其制造技术在聚合物功能化和支架结构方面具有多样性。本研究考虑开发聚（ε-己内酯）（PCL）免疫兼容且可生物降解的电纺支架，其包含头孢呋辛（CF）或二氧化钛（TiO₂）活性成分，既能提供针对眼部感染的杀菌活性，又能在体外支持角膜缘干细胞的生长。PCL/CF支架通过共混电纺制备，而TiO₂颗粒的功能化通过超声后处理进行。分别通过扫描电子显微镜、水接触角测量、拉伸测试和动态力学分析、抗菌测试以及紫外可见光谱，对制备的支架在物理结构、润湿性、静态和动态力学行为、抗菌效率和药物释放方面进行了评估。从人尸体角膜手术剩余物中分离出的人角膜缘干细胞，在PCL/CF和PCL/TiO₂支架上培养，并通过免疫细胞化学进一步鉴定细胞类型，从而针对角膜缘干细胞的p63标记物（一种核转录因子）和细胞角蛋白3（CK3，一种角膜上皮分化标记物）进行染色。电纺的PCL/CF和PCL/TiO₂成功支持了培养的角膜缘细胞的黏附、增殖和分化，并对……提供了抗菌效果
Zotero 插件
