Therapeutic Targeting Research Group, University of Newcastle, Callaghan, NSW, Australia; School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, Australia.
School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, Australia.
Int J Pharm. 2020 Oct 15;588:119739. doi: 10.1016/j.ijpharm.2020.119739. Epub 2020 Aug 9.
A key consideration in the clinical translation of nanomedicines is determining their in vivo biodistribution in preclinical studies, which is important for predicting and correlating therapeutic efficacy and safety. There are a number of techniques available for analyzing the in vivo biodistribution of nanoparticles, with each having its own advantages and limitations. However, conventional techniques are limited by their inability to image the three-dimensional (3D) association of nanoparticles with cells, vasculature and other biological structures in whole organs at a subcellular level. Recently, optical clearing techniques have been used to evaluate the biodistribution of nanoparticles by 3D organ imaging. Optical clearing is a procedure that is increasingly being used to improve the imaging of biological tissues, whereby light scattering substances are removed to better match the refractive indices of different tissue layers. The use of optical clearing techniques has the potential to transform the way we evaluate the biodistribution of new and existing nanomedicines, as it allows the visualization of the interaction of nanoparticles with the biological environment in intact tissues. This review will compare the main optical clearing techniques and will address the considerations for the use of these techniques to evaluate nanoparticle biodistribution.
在将纳米药物转化为临床应用时，一个关键的考虑因素是确定其在临床前研究中的体内生物分布，这对于预测和关联治疗效果和安全性非常重要。有许多技术可用于分析纳米颗粒的体内生物分布，每种技术都有其自身的优点和局限性。然而，传统技术受到其无法在亚细胞水平上对整个器官中纳米颗粒与细胞、血管和其他生物结构的三维关联进行成像的限制。最近，光学透明技术已被用于通过 3D 器官成像来评估纳米颗粒的生物分布。光学透明是一种越来越多地用于改善生物组织成像的方法，通过去除光散射物质，以更好地匹配不同组织层的折射率。光学透明技术的使用有可能改变我们评估新型和现有纳米药物生物分布的方式，因为它允许在完整组织中可视化纳米颗粒与生物环境的相互作用。这篇综述将比较主要的光学透明技术，并讨论使用这些技术评估纳米颗粒生物分布的注意事项。
