Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
Gene. 2020 Dec 30;763:144997. doi: 10.1016/j.gene.2020.144997. Epub 2020 Aug 9.
The CRISPR-Cas system currently stands as one of the best multifaceted tools for site-specific genome engineering in mammals. An important aspect of research in this field focusses on improving the specificity and efficacy of precise genome editing in multiple model systems. The cornerstone of this mini-review is one of the extensively investigated small molecule inhibitor, SCR7, which abrogates NHEJ, a Ligase IV-dependent DSB repair pathway, thus guiding integration of the foreign DNA fragment via the more precise homology directed repair during genome editing. One of our recent studies sheds light on properties of different forms of SCR7. Here, we give a succinct account on the use of SCR7 and its different forms in CRISPR-Cas system, highlighting their chemical properties and biological relevance as potent efficiency-enhancing CRISPR tools.
CRISPR-Cas 系统目前是哺乳动物中用于特定基因组工程的最佳多功能工具之一。该领域研究的一个重要方面集中在提高多种模型系统中精确基因组编辑的特异性和效率上。本篇综述的基石之一是一种经过广泛研究的小分子抑制剂 SCR7，它可以阻断非同源末端连接（NHEJ），这是一种依赖 Ligase IV 的 DSB 修复途径，从而在基因组编辑过程中通过更精确的同源定向修复引导外源 DNA 片段的整合。我们最近的一项研究揭示了不同形式的 SCR7 的特性。在这里，我们简要介绍了 SCR7 及其不同形式在 CRISPR-Cas 系统中的应用，强调了它们作为有效的 CRISPR 工具的化学性质和生物学相关性。
