Wang Sutian, Qu Zixiao, Huang Qiuyan, Zhang Jianfeng, Lin Sen, Yang Yecheng, Meng Fanming, Li Jianhao, Zhang Kunli
State Key Laboratory of Livestock and Poultry Breeding, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of Livestock Disease Prevention Guangdong Province, Guangzhou 510640, China.
Life (Basel). 2022 Jul 18;12(7):1070. doi: 10.3390/life12071070.
As a new genetic engineering technology, gene editing can precisely modify the specific gene sequence of the organism's genome. In the last 10 years, with the rapid development of gene editing technology, zinc-finger nucleases (ZFNs), transcription activator-like endonucleases (TALENs), and CRISPR/Cas9 systems have been applied to modify endogenous genes in organisms accurately. Now, gene editing technology has been used in mice, zebrafish, pigs, cattle, goats, sheep, rabbits, monkeys, and other species. Breeding for disease-resistance in agricultural animals tends to be a difficult task for traditional breeding, but gene editing technology has made this easier. In this work, we overview the development and application of gene editing technology in the resistance breeding of livestock. Also, we further discuss the prospects and outlooks of gene editing technology in disease-resistance breeding.
作为一种新的基因工程技术，基因编辑可以精确修饰生物体基因组的特定基因序列。在过去十年中，随着基因编辑技术的迅速发展，锌指核酸酶（ZFNs）、转录激活样效应物核酸酶（TALENs）和CRISPR/Cas9系统已被应用于精确修饰生物体内的内源基因。如今，基因编辑技术已应用于小鼠、斑马鱼、猪、牛、山羊、绵羊、兔子、猴子和其他物种。对农业动物进行抗病育种对传统育种来说往往是一项艰巨的任务，但基因编辑技术使这一过程变得更加容易。在这项工作中，我们概述了基因编辑技术在牲畜抗性育种中的发展和应用。此外，我们还进一步讨论了基因编辑技术在抗病育种中的前景和展望。
