Perdices Lorena, Fuentes-Broto Lorena, Segura Francisco, Cuenca Nicolás, Orduna-Hospital Elvira, Pinilla Isabel
Aragon Institute for Health Research (IIS Aragón), 50009 Zaragoza, Spain.
Department of Pharmacology, Physiology and Legal and Forensic Medicine, Universidad de Zaragoza, 50009 Zaragoza, Spain.
Antioxidants (Basel). 2020 Aug 8;9(8):718. doi: 10.3390/antiox9080718.
Retinitis pigmentosa (RP) includes a group of genetic disorders that involve the loss of visual function due to mutations mainly in photoreceptors but also in other retinal cells. Apoptosis, retinal disorganization, and inflammation are common in the progression of the disease. Epigallocatechin gallate (EGCG) has been proved as beneficial in different eye diseases. Pigmented heterozygous P23H rat was used as an animal model of RP. Visual function was assessed by optomotor and electroretinogram (ERG) and circadian rhythms were evaluated by telemetry. Hepatic oxidative damage and antioxidant defenses were assessed using biochemical tests. The visual function of the EGCG P23H group was preserved, with a deterioration in the activity period and lower values in the interdaily stability parameter. Control rats treated with EGCG were less active than the sham group. EGCG increased antioxidant levels in P23H rats but reduced total hepatic antioxidant capacity by almost 42% in control rats compared to the sham group. We conclude that treatment with EGCG improves visual function and antioxidant status in P23H rats but diminishes antioxidant defenses in wild-type control animals, and slightly worsens activity circadian rhythms. Further studies are necessary to clarify the beneficial effects in disease conditions and in healthy organisms.
视网膜色素变性（RP）包括一组遗传性疾病，这些疾病主要由于光感受器以及其他视网膜细胞中的突变导致视觉功能丧失。细胞凋亡、视网膜结构紊乱和炎症在该疾病的进展过程中很常见。表没食子儿茶素没食子酸酯（EGCG）已被证明对不同的眼部疾病有益。色素杂合P23H大鼠被用作RP的动物模型。通过视动反射和视网膜电图（ERG）评估视觉功能，并通过遥测评估昼夜节律。使用生化测试评估肝脏氧化损伤和抗氧化防御能力。EGCG处理的P23H组的视觉功能得以保留，活动期有所恶化，日间稳定性参数值较低。用EGCG处理的对照大鼠比假手术组的活动少。EGCG提高了P23H大鼠的抗氧化水平，但与假手术组相比，对照大鼠的肝脏总抗氧化能力降低了近42%。我们得出结论，EGCG治疗可改善P23H大鼠的视觉功能和抗氧化状态，但会削弱野生型对照动物的抗氧化防御能力，并使活动昼夜节律略有恶化。需要进一步研究以阐明其在疾病状态和健康生物体中的有益作用。
