Zhao Lianghui, Wang Hongwei, Feng Chengcheng, Song Fangying, Du Xianli
Qingdao Eye Hospital of Shandong First Medical University, Qingdao, China.
State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Qingdao, China.
Front Bioeng Biotechnol. 2021 Nov 16;9:759303. doi: 10.3389/fbioe.2021.759303. eCollection 2021.
Microbial infections caused by wearing contact lenses has become a major health problem, so the design and development of antibacterial contact lenses has attracted widespread attention. To safely and effectively inhibit bacterial adhesion of contact lenses, we have facilely prepared epigallocatechin gallate (EGCG) loaded starch hydrogel/contact lens composites by free radical polymerization of the mixture containing 2-hydroxylethyl methacrylate, methacrylic acid and ethylene glycol dimethacrylate. The adequate transmittance of the resulting contact lenses was characterized by ultraviolet-visible spectrophotometry, and their satisfactory stability was examined using differential scanning calorimetry and thermogravimetric analysis. Whereafter, cytotoxicity and degradation experiments were performed to investigate the biocompatibility and degradability of the contact lenses. The results showed the nontoxicity and good degradability of the composites. Besides, the capacity of the contact lenses for release of EGCG was also evaluated, and the results showed that the EGCG in these contact lenses can be sustainably released for at least 14 days. Further bacterial adhesion assay suggested that the EGCG loaded starch hydrogel/contact lenses could significantly reduce the adhesion of compared to the control. The EGCG loaded starch hydrogel/contact lens composites provide a potential intervention strategy for preventing ocular microbial infections and inhibiting bacterial keratitis.
佩戴隐形眼镜引起的微生物感染已成为一个主要的健康问题，因此抗菌隐形眼镜的设计与开发受到了广泛关注。为了安全有效地抑制隐形眼镜上的细菌黏附，我们通过对含有甲基丙烯酸羟乙酯、甲基丙烯酸和乙二醇二甲基丙烯酸酯的混合物进行自由基聚合反应，简便地制备了负载表没食子儿茶素没食子酸酯（EGCG）的淀粉水凝胶/隐形眼镜复合材料。通过紫外可见分光光度法对所得隐形眼镜的透光率进行了表征，并使用差示扫描量热法和热重分析对其令人满意的稳定性进行了检测。随后，进行了细胞毒性和降解实验，以研究隐形眼镜的生物相容性和可降解性。结果表明该复合材料无毒且具有良好的可降解性。此外，还评估了隐形眼镜释放EGCG的能力，结果表明这些隐形眼镜中的EGCG可以持续释放至少14天。进一步的细菌黏附试验表明，与对照组相比，负载EGCG的淀粉水凝胶/隐形眼镜能够显著降低细菌的黏附。负载EGCG的淀粉水凝胶/隐形眼镜复合材料为预防眼部微生物感染和抑制细菌性角膜炎提供了一种潜在的干预策略。
