Loubser Johannes, Hills Paul
Institute for Plant Biotechnology, Department of Genetics, Faculty of AgriSciences, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa.
Plants (Basel). 2020 Aug 10;9(8):1010. doi: 10.3390/plants9081010.
The aim of this study was to assess the effect of BC204 as a plant biostimulant on plants under normal and NaCl-stressed conditions.
For this study, ex vitro and in vitro growth experiments were conducted to assess the effect of both NaCl and BC204 on basic physiological parameters such as biomass, chlorophyll, proline, malondialdehyde, stomatal conductivity, Fv/Fm and the expression of four NaCl-responsive genes.
This study provides preliminary evidence that BC204 mitigates salt stress in . BC204 treatment increased chlorophyll content, fresh and dry weights, whilst reducing proline, anthocyanin and malondialdehyde content in the presence of 10 dS·m electroconductivity (EC) salt stress. Stomatal conductivity was also reduced by BC204 and NaCl in source leaves. In addition, BC204 had a significant effect on the expression of salinity-related genes, stimulating the expression of salinity-related genes and independently of NaCl-stress.
BC204 stimulated plant growth under normal growth conditions by increasing above-ground shoot tissue and root and shoot growth in vitro. BC204 also increased chlorophyll content while reducing stomatal conductivity. BC204 furthermore mitigated moderate to severe salt stress (10-20 dS·m) in . Under salt stress conditions, BC204 reduced the levels of proline, anthocyanin and malondialdehyde. The exact mechanism by which this occurs is unknown, but the results in this study suggest that BC204 may act as a priming agent, stimulating the expression of genes such as and .
本研究旨在评估BC204作为一种植物生物刺激素在正常和NaCl胁迫条件下对植物的影响。
在本研究中，进行了离体和体外生长实验，以评估NaCl和BC204对基本生理参数的影响，如生物量、叶绿素、脯氨酸、丙二醛、气孔导度、Fv/Fm以及四个NaCl响应基因的表达。
本研究提供了初步证据，表明BC204可减轻盐胁迫。在10 dS·m电导率（EC）盐胁迫下，BC204处理增加了叶绿素含量、鲜重和干重，同时降低了脯氨酸、花青素和丙二醛含量。BC204和NaCl还降低了源叶的气孔导度。此外，BC204对盐度相关基因的表达有显著影响，在不依赖NaCl胁迫的情况下刺激了盐度相关基因和的表达。
BC204在正常生长条件下通过增加地上茎组织以及离体培养中的根和茎生长来刺激植物生长。BC204还增加了叶绿素含量，同时降低了气孔导度。此外，BC204减轻了盐胁迫。在盐胁迫条件下，BC204降低了脯氨酸、花青素和丙二醛的水平。其发生的确切机制尚不清楚，但本研究结果表明，BC204可能作为一种引发剂，刺激和等基因的表达。
