Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, P. R. China.
J Mater Chem B. 2020 Sep 23;8(36):8282-8293. doi: 10.1039/d0tb01562h.
Hemorrhage remains one of the direct causes of high mortality. The development of ideal hemostatic materials with sound ability to deal with severe wound is urgent needed. Although starch-based hemostatic powder has been widely used, hydrous physiological environments severely hamper its binding to the target tissue, thereby limiting the effectiveness in hemostasis. Herein, inspired by mussel adhesive protein, a novel injectable tissue-adhesive hydrogel (St-Dopa hydrogel) composed of starch, succinic anhydride and dopamine was developed in situ by enzymatic crosslinking. The results show that St-Dopa hydrogels were intimately integrated with biological tissue and formed robust barriers to reduce blood loss. St-Dopa hydrogels exhibited superior capacity for in vitro and in vivo hemostasis as compared with chitin hydrogels. In addition to the ease of operation, St-Dopa hydrogels exhibited rapid sol-gel transition, porous microscopic morphology, good swelling ratio and biodegradability, tissue-like elastomeric mechanical properties and excellent cyto/hemo-compatibility. These results suggest that this newly developed St-Dopa hydrogel is a promising biological adhesive and hemostatic material.
出血仍然是高死亡率的直接原因之一。急需开发具有良好能力来应对严重伤口的理想止血材料。尽管基于淀粉的止血粉已被广泛应用，但含水的生理环境严重阻碍了其与目标组织的结合，从而限制了其在止血方面的效果。受贻贝类黏附蛋白的启发，本文通过酶交联原位制备了由淀粉、琥珀酸酐和多巴胺组成的新型可注射组织黏附水凝胶（St-Dopa 水凝胶）。结果表明，St-Dopa 水凝胶与生物组织紧密结合，形成坚固的屏障以减少失血。与壳聚糖水凝胶相比，St-Dopa 水凝胶在体外和体内止血方面表现出更好的性能。除了操作简便外，St-Dopa 水凝胶还具有快速溶胶-凝胶转变、多孔微观形貌、良好的溶胀比和生物降解性、类组织弹性机械性能和优异的细胞/血液相容性。这些结果表明，这种新开发的 St-Dopa 水凝胶是一种很有前途的生物黏附剂和止血材料。
