College of Marine Life Science, Ocean University of China, 5 Yushan Road, 266003 Qingdao, China.
The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266003, China.
Carbohydr Polym. 2020 Jun 1;237:116138. doi: 10.1016/j.carbpol.2020.116138. Epub 2020 Mar 6.
Schiff base reaction crosslinking hydrogels are advantageous by rapid formation and absence of external crosslinkers. However, poor mechanical hindered their broader applications. Here, a mechanically strengthened tissue adhesive was constructed through incorporation of chitin nano-whiskers (CtNWs) with a Schiff base crosslinking hydrogel of carboxymethyl chitosan (CMCS) and dextran dialdehyde (DDA). The optimal formulation of complexed hydrogel exhibited 1.87 folds higher compressive stress than non-complexed and 1.51 time higher adhesive strength on porcine skin. The complexed hydrogel exhibited negligible cytotoxicity, anti-swelling performance in PBS, optimum antibacterial and hemostatic capacities. In vivo implantation studies confirmed the complexed hydrogel was degradable without long-term inflammatory responses. Desirable efficacy of injectable complexed hydrogel as hemostat was demonstrated in rat liver injury model, which could avoid severe postoperative adhesion and necrosis as observed in the treatment with commercial 3 M™ vetbond™ tissue adhesive. The results highlighted that the complexed hydrogel potentiated rapid hemostasis and wound repair applications.
席夫碱反应交联水凝胶具有快速形成和无需外部交联剂的优点。然而，较差的机械性能限制了它们的更广泛应用。在这里，通过将壳聚糖纳米纤维（CtNWs）与壳聚糖羧甲基（CMCS）和葡聚糖二醛（DDA）的席夫碱交联水凝胶结合，构建了一种机械强化的组织粘合剂。复合水凝胶的最佳配方表现出比非复合水凝胶高 1.87 倍的压缩应力，比非复合水凝胶高 1.51 倍的对猪皮的粘附强度。复合水凝胶表现出可忽略不计的细胞毒性、在 PBS 中的抗肿胀性能、最佳的抗菌和止血能力。体内植入研究证实，复合水凝胶可降解，无长期炎症反应。在大鼠肝损伤模型中，可注射复合水凝胶作为止血剂的效果令人满意，可避免在使用商业 3M™vetbond™组织粘合剂治疗时观察到的严重术后粘连和坏死。结果表明，复合水凝胶增强了快速止血和伤口修复应用。
