Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, No.127 Youyi West Road, Xi'an, Shaanxi 710072, People's Republic of China.
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, No.127 Youyi West Road, Xi'an, Shaanxi 710072, People's Republic of China.
Int J Biol Macromol. 2021 Jul 31;183:2142-2151. doi: 10.1016/j.ijbiomac.2021.05.147. Epub 2021 May 25.
As a kind of natural protein derived material, gelatin has been widely used in the preparation of medical hydrogels due to its good biocompatibility, non-immunogenicity and the ability of promoting cell adhesion. Functionalization of gelatin-based hydrogels is a hot topic in research and its clinic application. Herein, a novel gelatin-based adhesive hydrogel was prepared via mussel-inspired chemistry. Gelatin was firstly functionalized by dopamine to form dopamine grafted gelatin (GelDA). After the mixture with 1,4-phenylenebisboronic acid and graphene oxide (GO), the GelDA/GO hydrogels were obtained by HO/HRP (horseradish peroxidase) catalytic system. Based on the self-healing and tissue adhesion of the hydrogels, the hemostatic property has been exhibited in the rat hepatic hemorrhage model. Additionally, the incorporation of GO endowed conductivity and enhanced the mechanical property of GelDA/GO hydrogels. The electromyography (EMG) signals of finger movement were successfully monitored by using hydrogel as the adhesive electrodes of EMG monitor. L929 cell experiments showed that the hydrogels had good cytocompatibility. The results indicated the potential application of GelDA/GO hydrogels in tissue adhesives, wound dressings, and wearable devices.
作为一种天然蛋白质衍生材料，明胶由于其良好的生物相容性、非免疫原性和促进细胞黏附的能力，已被广泛应用于医学水凝胶的制备。基于明胶的水凝胶的功能化是研究的热点，也是其临床应用的热点。本文通过贻贝启发的化学方法制备了一种新型的基于明胶的黏附水凝胶。首先通过多巴胺对明胶进行功能化，得到多巴胺接枝明胶（GelDA）。然后与 1,4-亚苯基二硼酸和氧化石墨烯（GO）混合，在 HO/HRP（辣根过氧化物酶）催化体系下得到 GelDA/GO 水凝胶。基于水凝胶的自修复和组织黏附性能，在大鼠肝出血模型中表现出了止血性能。此外，GO 的加入赋予了 GelDA/GO 水凝胶导电性，并增强了其机械性能。水凝胶作为肌电监测器的黏附电极，成功监测到手指运动的肌电图（EMG）信号。L929 细胞实验表明，水凝胶具有良好的细胞相容性。结果表明，GelDA/GO 水凝胶在组织黏合剂、伤口敷料和可穿戴设备中有潜在的应用。
