Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea.
Department of Medicine, Graduate School, Chung-Ang University, Seoul, Korea.
Skin Res Technol. 2021 Jan;27(1):5-14. doi: 10.1111/srt.12899. Epub 2020 Aug 12.
Non-invasive body-sculpting procedures are becoming increasingly popular. The application of 1,060 nm of laser energy transcutaneously to hyperthermically induce the disruption of fat cells in the abdomen is a type of non-invasive procedure.
The purpose of this study was to compare the treatment results from two parameters of the same system, each with different energy output levels, in an in vivo porcine model to determine the most effective application.
Female pigs (n = 3) were used in this study. We examined the effects of the treatment using photography, ultrasonography, gross and microscopic pathology, and histological examination in order to determine the mechanism of action, efficacy, and safety of the procedure. Blood chemistry analysis was performed before each session to check lipid levels and to monitor for any adverse changes in markers that may indicate liver damage. Biopsies were taken and routinely processed with hematoxylin and eosin and Oil Red O stains to examine for tissue damage at baseline and after each treatment. Terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling (TUNEL) assays were performed to check for apoptotic-related DNA damage.
Ultrasonic imaging of the same area before and after the application of 1,060 nm of laser energy at outputs of 0.9 and 1.4 W/cm showed that the density of the fat layer changed immediately after irradiation due to the transient heat transfer in the fat layer. Preclinical evaluation was performed to obtain comparison data on the safety and efficacy of subcutaneous fat reduction after applying the different energy outputs of 0.9 and 1.4 W/cm .
Based on our findings, we suggest that long-term histologic changes through the use of these devices suggest a comparative effectiveness of the treatment energy.
非侵入式身体塑形程序越来越受欢迎。将 1060nm 的激光能量经皮应用以产生热，从而破坏腹部的脂肪细胞，这是一种非侵入性程序。
本研究的目的是比较同一系统的两个参数的治疗结果，每个参数的能量输出水平不同，在体内猪模型中以确定最有效的应用。
本研究使用雌性猪（n=3）。我们通过摄影、超声、大体和显微镜病理学以及组织学检查来检查治疗效果，以确定该程序的作用机制、疗效和安全性。在每次治疗前进行血液化学分析，以检查血脂水平，并监测可能表明肝损伤的任何标记物的不良变化。采集活检并常规用苏木精和伊红以及油红 O 染色处理，以检查基线和每次治疗后的组织损伤。进行末端脱氧核苷酸转移酶脱氧尿苷三磷酸末端标记（TUNEL）测定，以检查与凋亡相关的 DNA 损伤。
在应用 0.9 和 1.4W/cm 的 1060nm 激光能量前后对同一区域进行超声成像显示，由于脂肪层中的瞬态热传递，脂肪层的密度在照射后立即发生变化。进行临床前评估以获得应用 0.9 和 1.4W/cm 的不同能量输出后皮下脂肪减少的安全性和疗效的比较数据。
根据我们的发现，我们建议长期使用这些设备的组织学变化表明治疗能量具有可比性。
