ICMA, Institute of Materials Science of Aragon, CSIC, University of Zaragoza, 50008 Zaragoza, Spain.
Phantom-g, CICECO-Aveiro Institute of Materials, Department of Physics, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
Nano Lett. 2020 Sep 9;20(9):6466-6472. doi: 10.1021/acs.nanolett.0c02163. Epub 2020 Aug 4.
Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln-bearing polymeric micellar probes (Ln = Sm, Eu) incubated in breast cancer MDA-MB468 cells. Two-dimensional (2D) thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to ∼20 degrees and subcellular gradients of ∼5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes.
由于生化环境（如 pH 值和离子强度）的复杂性以及细胞内快速但尚未很好理解的热传递机制，测量单个细胞中的产热是一项极具挑战性的任务。在这里，我们提出了一种使用合理设计的发光 Ln 配合聚合物胶束探针（Ln = Sm，Eu）在荧光显微镜下进行细胞内温度测绘的独特系统（不确定性为 0.2 K），这些探针在乳腺癌 MDA-MB468 细胞中孵育。记录的二维（2D）热图像显示，当细胞培养液的温度在 296 和 304 K 之间升高时，细胞内的温度呈现不均匀的进展，最高可达 20 度，核仁与细胞其余部分之间的亚细胞梯度约为 5 度，这说明了不同细胞器的产热活性，并突出了该工具研究细胞内过程的潜力。
