Ye Ziyang, Harrington Benjamin, Pickel Andrea D
Materials Science Program, University of Rochester, Rochester, NY 14627, USA.
Department of Mechanical Engineering, University of Rochester, Rochester, NY 14627, USA.
Sci Adv. 2024 Jul 19;10(29):eado6268. doi: 10.1126/sciadv.ado6268. Epub 2024 Jul 17.
From engineering improved device performance to unraveling the breakdown of classical heat transfer laws, far-field optical temperature mapping with nanoscale spatial resolution would benefit diverse areas. However, these attributes are traditionally in opposition because conventional far-field optical temperature mapping techniques are inherently diffraction limited. Optical super-resolution imaging techniques revolutionized biological imaging, but such approaches have yet to be applied to thermometry. Here, we demonstrate a super-resolution nanothermometry technique based on highly doped upconverting nanoparticles (UCNPs) that enable stimulated emission depletion (STED) super-resolution imaging. We identify a ratiometric thermometry signal and maintain imaging resolution better than ~120 nm for the relevant spectral bands. We also form self-assembled UCNP monolayers and multilayers and implement a detection scheme with scan times >0.25 μm/min. We further show that STED nanothermometry reveals a temperature gradient across a joule-heated microstructure that is undetectable with diffraction limited thermometry, indicating the potential of this technique to uncover local temperature variation in wide-ranging practical applications.
从提高器件性能到揭示经典热传递定律的失效，具有纳米级空间分辨率的远场光学温度映射将使多个领域受益。然而，这些特性传统上是相互矛盾的，因为传统的远场光学温度映射技术本质上受衍射限制。光学超分辨率成像技术彻底改变了生物成像，但此类方法尚未应用于温度测量。在此，我们展示了一种基于高掺杂上转换纳米粒子（UCNP）的超分辨率纳米温度测量技术，该技术能够实现受激发射损耗（STED）超分辨率成像。我们识别出一种比率温度测量信号，并在相关光谱波段保持优于约120 nm的成像分辨率。我们还形成了自组装的UCNP单层和多层结构，并实施了扫描速度>0.25 μm/分钟的检测方案。我们进一步表明，STED纳米温度测量揭示了焦耳加热微结构上的温度梯度，而衍射受限温度测量无法检测到该梯度，这表明该技术在广泛的实际应用中揭示局部温度变化的潜力。
