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天府黑兔肌肉品质性状相关 mRNA 表达的关联分析

Association of mRNA expression with meat quality trait in Tianfu black rabbits.

机构信息

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.

出版信息

Anim Biotechnol. 2022 Jun;33(3):480-486. doi: 10.1080/10495398.2020.1804920. Epub 2020 Aug 13.

Abstract

is a hormone synthesized and secreted primarily in adipocyte which can help to regulate energy balance. In this experiment, three tissue samples of Tianfu black rabbits at four growing periods were selected. The expression levels of gene in different tissues were detected by real-time fluorescence quantitative PCR. The correlation analysis showed that the correlation coefficient between the expression levels of gene in perirenal fat and intramuscular fat content in 84-day-old male rabbits was 0.73 ( < 0.05); the correlation coefficients between the expression levels of gene in left biceps femoris and intramuscular fat and 24-hour pH in 84-day-old male rabbits were 0.95 ( < 0.01) and 0.85 ( < 0.05), respectively. Besides, the correlation coefficient between the expression levels of gene in male left biceps femoris and cooked meat rate was 0.83 ( < 0.05). According to the analysis results, we inferred the expression levels of gene in Tianfu black rabbits can influence meat quality and the meat quality of high expression levels of gene in Tianfu black rabbits is better. These results revealed the gene may be one of the important candidate genes for meat quality traits of molecular markers.

摘要

是一种主要在脂肪细胞中合成和分泌的激素,有助于调节能量平衡。本实验选用天府黑兔四个生长阶段的三种组织样品,通过实时荧光定量 PCR 检测不同组织中 基因的表达水平。相关性分析表明,84 日龄雄性兔肾周脂肪 基因表达水平与肌肉内脂肪含量的相关系数为 0.73(<0.05);左股二头肌 基因表达水平与肌肉内脂肪和 84 日龄雄性兔 24 小时 pH 的相关系数分别为 0.95(<0.01)和 0.85(<0.05);此外,雄性左股二头肌 基因表达水平与熟肉率的相关系数为 0.83(<0.05)。根据分析结果推断,天府黑兔 基因的表达水平可能影响肉质,高表达水平的天府黑兔的肉质更好。这些结果表明,该基因可能是肉质分子标记的重要候选基因之一。

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