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Excision of apurinic and/or apyrimidinic sites from DNA by nucleolytical enzymes from rat brain.

作者信息

Ivanov V A, Tretyak T M, Afonin Y N

机构信息

Institute of Biological Physics, Academy of Sciences of the USSR, Pushchino.

出版信息

Eur J Biochem. 1988 Feb 15;172(1):155-9. doi: 10.1111/j.1432-1033.1988.tb13867.x.

DOI:10.1111/j.1432-1033.1988.tb13867.x
PMID:3278904
Abstract

Apurinic and/or apyrimidinic (AP) sites were excised from PM2 phage DNA by two enzymes: an AP endodeoxyribonuclease isolated from rat neocortex chromatin and a rat brain exodeoxyribonuclease, DNase B III. The resulting gap was filled with DNA polymerase beta prepared from rat liver and finally ligated by Escherichia coli DNA ligase.

摘要

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Genomic damage and its repair in young and aging brain.年轻及衰老大脑中的基因组损伤及其修复
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Purification and characterization of a deoxy-ribonuclease acting on native and UV irradiated DNA from young and aging rat brain.一种作用于幼年和老年大鼠大脑天然及紫外线照射后DNA的脱氧核糖核酸酶的纯化与特性分析
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