Excision of apurinic and/or apyrimidinic sites from DNA by nucleolytical enzymes from rat brain.
作者信息
Ivanov V A, Tretyak T M, Afonin Y N
机构信息
Institute of Biological Physics, Academy of Sciences of the USSR, Pushchino.
出版信息
Eur J Biochem. 1988 Feb 15;172(1):155-9. doi: 10.1111/j.1432-1033.1988.tb13867.x.
Abstract
Apurinic and/or apyrimidinic (AP) sites were excised from PM2 phage DNA by two enzymes: an AP endodeoxyribonuclease isolated from rat neocortex chromatin and a rat brain exodeoxyribonuclease, DNase B III. The resulting gap was filled with DNA polymerase beta prepared from rat liver and finally ligated by Escherichia coli DNA ligase.
摘要
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