Bergui L, Tesio L, Schena M, Riva M, Malavasi F, Schulz T, Marchisio P C, Caligaris-Cappio F
Dipartimento di Scienze Biomediche e Oncologia Umana, Università di Torino, Italy.
Eur J Immunol. 1988 Jan;18(1):89-96. doi: 10.1002/eji.1830180114.
The modulation of surface molecules on B-chronic lymphocytic leukemia (B-CLL) cells was studied in vitro by incubation with CD19, CD20, CD21, CD5 and anti-IgM antibodies. The cytoplasmic changes were evaluated by analyzing the organization of cytoskeletal F-actin and the adhesive properties of stimulated B-CLL cells. The following observations were made: (a) CD5 antigen is capped on the surface of B-CLL cells (but not on normal CD5+ B and T lymphocytes). (b) On B-CLL cells, CD5 and CD21, the receptors for the C3d fraction of complement, co-cap and co-modulate while surface IgM as well as CD19 and CD20 do not cap. (c) B-CLL cells, after capping of CD5 or CD21 surface molecules, fail to organize F-actin into podosomes. (d) The pre-incubation of B-CLL cells with either CD5 or CD21 antibodies prevents their binding to purified C3d protein used to coat coverslips. These data indicate that an intimate spatial relationship exists between CD5 and CD21 molecules on the B-CLL surface. The CD5-CD21 complex is involved in the redistribution of cytoskeletal proteins which may control the adhesive properties of these malignant B cells.
通过与CD19、CD20、CD21、CD5和抗IgM抗体孵育，在体外研究了B细胞慢性淋巴细胞白血病（B-CLL）细胞表面分子的调节。通过分析细胞骨架F-肌动蛋白的组织和受刺激的B-CLL细胞的黏附特性来评估细胞质变化。得到以下观察结果：（a）CD5抗原在B-CLL细胞表面形成帽状（但在正常CD5+B和T淋巴细胞表面不形成帽状）。（b）在B-CLL细胞上，补体C3d片段的受体CD5和CD21共同形成帽状并共同调节，而表面IgM以及CD19和CD20不形成帽状。（c）CD5或CD21表面分子形成帽状后，B-CLL细胞无法将F-肌动蛋白组织成足体。（d）用CD5或CD21抗体预孵育B-CLL细胞可阻止它们与用于包被盖玻片的纯化C3d蛋白结合。这些数据表明B-CLL表面的CD5和CD21分子之间存在密切的空间关系。CD5-CD21复合物参与细胞骨架蛋白的重新分布，这可能控制这些恶性B细胞的黏附特性。
