Roth M, Boyle J M, Müller H
Dept of Research, University Clinics, Kantonsspital, Basel, Switzerland.
Experientia. 1988 Feb 15;44(2):169-71. doi: 10.1007/BF01952205.
Dysplastic naevus syndrome (DNS) is frequently observed in association with familial melanoma and xeroderma pigmentosum (XP), but the role of UV-light in the development of DNS has not been elucidated. Previous work has shown that UV-induced unscheduled DNA synthesis is associated with the early loss of antigenicity observed in immunoassays using a monoclonal antibody specific for thymine-thymine dimers. We now show that the rate of loss of antigenicity, which reflects the relative amount of bound antibody, observed during the first 60 min following 10 Jm-2 UVC irradiation is significantly reduced (p = 0.02) in cultures of fibroblasts from 7 out of 8 DNS patients compared with the results from cells of a group of 30 healthy volunteers. This observation suggests an early event in excision repair is altered in the majority of DNS patients.
发育异常痣综合征（DNS）常与家族性黑色素瘤和着色性干皮病（XP）相关，但紫外线在DNS发生发展中的作用尚未阐明。先前的研究表明，紫外线诱导的非程序性DNA合成与在使用针对胸腺嘧啶 - 胸腺嘧啶二聚体的单克隆抗体进行的免疫测定中观察到的抗原性早期丧失有关。我们现在表明，与30名健康志愿者细胞的结果相比，在10 Jm-2 UVC照射后的最初60分钟内观察到的抗原性丧失率（反映结合抗体的相对量）在8名DNS患者中的7名患者的成纤维细胞培养物中显著降低（p = 0.02）。这一观察结果表明，大多数DNS患者的切除修复早期事件发生了改变。
