与链霉素抗性突变体的S12核糖体蛋白中特定氨基酸变化相关的大肠杆菌表面性质改变。
Altered surface properties of Escherichia coli associated with a specific amino acid change in the S12 ribosomal protein of streptomycin-resistant mutants.
作者信息
Pestka S, Walter H, Wayne L G
出版信息
Antimicrob Agents Chemother. 1977 Jun;11(6):978-83. doi: 10.1128/AAC.11.6.978.
Escherichia coli mutants resistant to streptomycin exhibited differences in countercurrent distribution from the parental strains. The degree of difference from the parental strain correlated with the degree of restriction of translation and thus the particular strA allele. The changes in countercurrent distribution in the phase systems used probably resulted predominantly from surface charge alterations. The differences in countercurrent distribution in these and other mutants may be a useful selective technique to obtain different types of mutants for which specific selective techniques may not be available. In addition, it appears that the surface properties of cells, which determine their position in countercurrent distribution, are a function of the translational efficiency and fidelity, and that the surface of cells consists of a mosaic that is an expression of this translational fidelity.
对链霉素具有抗性的大肠杆菌突变体在逆流分布上与亲本菌株存在差异。与亲本菌株的差异程度与翻译受限程度相关，进而与特定的strA等位基因相关。在所使用的相系统中，逆流分布的变化可能主要是由表面电荷改变引起的。这些突变体以及其他突变体在逆流分布上的差异可能是一种有用的选择技术，用于获得可能没有特定选择技术的不同类型的突变体。此外，似乎决定细胞在逆流分布中位置的细胞表面特性是翻译效率和保真度的函数，并且细胞表面由一个镶嵌体组成，该镶嵌体是这种翻译保真度的一种表达。
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