Michel-Briand Y, Laporte J M, Dupont M J, Couetdic G
Laboratoire de Bactériologie-Virologie-Parasitologie, Faculté de Médecine et de Pharmaeie, Centre Hospitalier Régional et Universitaire, Besançon, France.
Pathol Biol (Paris). 1988 Feb;36(2):159-62.
The pathogenicity of some enterobacteria is due to a plasmid encoding for outer membrane proteins or for toxins. The elimination of the plasmid gives a non-virulent strain. We have tried to eliminate plasmids encoding for the thermostable toxin of Escherichia coli (plasmid pCSltl) or for the enteroinvasive property of Shigella (plasmids pWR24, pHW401, pWR110), of Salmonella (plasmid pSD6) and of Yersinia (plasmids pYL4 and P4). The loss of plasmid was confirmed by agarose gel electrophoresis of a crude lysate. Fourteen antibiotics belonging to different chemical families were used at subinhibitory concentration. A control experiment without antibiotic was carried out to detect spontaneous loss of plasmids. No antibiotic was able to eliminate plasmids pCSltl, pHW401, pSD6, pYL4. Novobiocin eliminated pWR24 and pWR110, rifampicin eliminated pWR110 and P4. Three other antibiotics gave a cure of bacteria harbouring plasmids pWR110 and P4 but the percentage of cure was too low for a therapeutical interest.
一些肠道杆菌的致病性归因于编码外膜蛋白或毒素的质粒。去除质粒会产生无毒菌株。我们试图去除编码大肠杆菌热稳定毒素的质粒（质粒pCSltl）或志贺氏菌（质粒pWR24、pHW401、pWR110）、沙门氏菌（质粒pSD6）和耶尔森氏菌（质粒pYL4和P4）的侵袭性的质粒。通过粗裂解物的琼脂糖凝胶电泳确认质粒的丢失。使用了属于不同化学家族的14种抗生素，浓度为亚抑制浓度。进行了无抗生素的对照实验以检测质粒的自发丢失。没有抗生素能够去除质粒pCSltl、pHW401、pSD6、pYL4。新生霉素去除了pWR24和pWR110，利福平去除了pWR110和P4。其他三种抗生素治愈了携带质粒pWR110和P4的细菌，但治愈百分比过低，没有治疗价值。
