Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Science and Technology, Guangzhou 510650, China.
Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Bioresour Technol. 2020 Nov;316:123964. doi: 10.1016/j.biortech.2020.123964. Epub 2020 Aug 7.
Nitrogen-incorporating bacteria in activated sludge play important roles in nitrogen removal in sequencing bactch reactor (SBR), but the active microorganisms and their interactions in the complex community are rarely revealed. Herein, a combining cultivation-dependent and cultivation-independent methods associated with DNA-stable-isotope probing (SIP) was applied to determine the microbes responsible for nitrogen-incorporating in SBR. Results revealed that Cytophagaceae and Sphingobacteriales were identified to be involved in nitrification, and Anaerolineae, Plasticicumulans and Elusimicrobia were responsible for denitrification. Cultivable nitrobacter and denitrifiers were isolated from the activated sludge, but they did not participate in the nitrogen-incorporating based on the SIP results. Additionally, the molecular ecological network analysis indicated that the SIP-identified nitrogen-incorporating bacteria exhibited more links with the intra-community, which might explain the failure of isolating these active bacteria. These findings add understanding of the removal of nitrogenous compounds drived by nitrogen-incorporating bacteria in actual wastewater treatment process.
在序批式间歇反应器（SBR）中，含氮细菌在氮去除中起着重要作用，但复杂群落中活性微生物及其相互作用很少被揭示。本研究采用结合培养依赖和培养独立方法与 DNA 稳定同位素探针（SIP）相结合，以确定 SBR 中负责含氮的微生物。结果表明，Cytophagaceae 和 Sphingobacteriales 被鉴定为硝化作用的参与者，而 Anaerolineae、Plasticicumulans 和 Elusimicrobia 则负责反硝化作用。可培养的硝化菌和反硝化菌从活性污泥中分离出来，但根据 SIP 结果，它们并未参与含氮。此外，分子生态网络分析表明，SIP 鉴定的含氮细菌与群落内的联系更多，这可能解释了分离这些活性细菌的失败。这些发现增加了对实际废水处理过程中含氮细菌去除含氮化合物的理解。
