Food Security and Safety Niche Area, Faculty of Natural and Agricultural Sciences, North-West University, Mmabatho, South Africa.
Plant Signal Behav. 2021 Dec 2;16(12):1974217. doi: 10.1080/15592324.2021.1974217. Epub 2021 Sep 30.
Agrochemical applications on farmland aim to enhance crop yield; however, the consequence of biodiversity loss has caused a reduction in ecological functions. The positive endosphere interactions and crop rotation systems may function in restoring a stable ecosystem. Employing culture-independent techniques will help access the total bacteria community in the sunflower endosphere. Limited information is available on the bacteria diversity in sunflower plants cultivated under different agricultural practices. Hence, this study was designed to investigate the endophytic bacterial community structure of sunflower at the growing stage. Plant root and stem samples were sourced from two locations (Itsoseng and Lichtenburg), for DNA extraction and sequenced on the Illumina Miseq platform. The sequence dataset was analyzed using online bioinformatics tools. Saccharibacteria and Acidobacteria were dominant in plant roots, while the stem is dominated by Proteobacteria, Bacteriodetes, and Gemmatimonadetes across the sites. Bacterial genera, , and were found dominant in the root, while the stem is dominated by . The diverse bacterial community structure at phyla and class levels were significantly different in plant organs across the sites. The influence of soil physical and chemical parameters analyzed was observed to induce bacterial distribution across the sites. This study provides information on the dominant bacteria community structure in sunflowers at the growing stage and their predictive functions, which suggest their future exploration as bioinoculants for improved agricultural yields.
农田上的农用化学品应用旨在提高作物产量；然而，生物多样性丧失的后果导致生态功能下降。积极的根内共生相互作用和轮作制度可能有助于恢复稳定的生态系统。采用非培养技术将有助于获取向日葵根内总细菌群落。关于不同农业实践下种植的向日葵植物中细菌多样性的信息有限。因此，本研究旨在调查生长阶段向日葵的根内细菌群落结构。从两个地点（Itsoseng 和 Lichtenburg）采集植物根和茎样本，用于 DNA 提取并在 Illumina Miseq 平台上测序。使用在线生物信息学工具分析序列数据集。在根中占优势的是 Saccharibacteria 和 Acidobacteria，而在茎中占优势的是 Proteobacteria、Bacteriodetes 和 Gemmatimonadetes。在根中发现细菌属 、 和 占优势，而在茎中则以 占优势。在不同地点的植物器官中，细菌的门和纲水平的群落结构存在显著差异。分析观察到土壤物理和化学参数的影响会导致细菌在不同地点的分布。本研究提供了生长阶段向日葵中优势细菌群落结构及其预测功能的信息，这表明它们未来可能作为生物接种剂用于提高农业产量。
