人诱导多能干细胞的化学定义和无动物血清的冷冻保存。

Chemically Defined and Xeno-Free Cryopreservation of Human-Induced Pluripotent Stem Cells.

机构信息

Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.

Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta University, Augusta, GA, USA.

出版信息

Methods Mol Biol. 2021;2180:569-579. doi: 10.1007/978-1-0716-0783-1_29.

DOI:10.1007/978-1-0716-0783-1_29

PMID:32797435

Abstract

Human-induced pluripotent stem cells (hiPSCs) can be derived from a variety of biopsy samples and have an unlimited capacity for self-renewal and differentiation into almost any cell type in the body. Therefore, hiPSCs offer unprecedented opportunities for patient-specific cell therapies, modeling of human diseases, biomarker discovery, and drug testing. However, clinical applications of hiPSCs require xeno-free and, ideally, chemically defined methods for their generation, expansion, and cryopreservation. In this chapter, we present a chemically defined and xeno-free slow freezing method for hiPSCs along with a chemically undefined protocol. Both approaches yield reasonable post-thaw viability and cell growth.

摘要

人诱导多能干细胞（hiPSCs）可以从多种活检样本中获得，具有无限的自我更新能力，并能分化为体内几乎任何类型的细胞。因此，hiPSCs 为患者特异性细胞治疗、人类疾病建模、生物标志物发现和药物测试提供了前所未有的机会。然而，hiPSCs 的临床应用需要无动物源且理想情况下是化学成分确定的方法来进行其生成、扩增和冷冻保存。在本章中，我们提出了一种化学成分确定和无动物源的慢冻法，以及一种化学成分不确定的方案。这两种方法都能得到合理的冻后存活率和细胞生长。