Department of Microorganisms - Cyanobacteria and Protists, Leibniz Institute - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Methods Mol Biol. 2021;2180:639-645. doi: 10.1007/978-1-0716-0783-1_34.
Plant cell cultures consist of single cells or cell clusters growing as callus or suspension. Such cell cultures may be able to produce secondary metabolites and/or possess embryogenic potential. Therefore, they can be used for very different purposes in research, biotechnological applications, as well as for plant propagation. Cryopreservation is the only practical method to preserve such cultures until they are needed. Different cryopreservation approaches that have been developed for differentiated plant tissues including slow freezing, vitrification, and encapsulation/dehydration have also been applied to plant cell cultures. The controlled rate or slow-freezing approach, however, remains to be the gold standard for cell cultures. In this chapter, a standard slow-freezing cryopreservation procedure in combination with alginate immobilization is presented for long-term preservation of plant cell cultures.
植物细胞培养物由生长为愈伤组织或悬浮液的单个细胞或细胞簇组成。这些细胞培养物可能能够产生次生代谢物和/或具有胚胎发生潜力。因此，它们可以在研究、生物技术应用以及植物繁殖中用于非常不同的目的。冷冻保存是保存这些培养物直到需要它们的唯一实用方法。已经为分化的植物组织开发了不同的冷冻保存方法，包括慢速冷冻、玻璃化和包封/脱水，也已应用于植物细胞培养物。然而，控制速率或慢速冷冻方法仍然是细胞培养物的金标准。在本章中，介绍了一种标准的慢速冷冻冷冻保存程序，结合海藻酸钙固定化，用于植物细胞培养物的长期保存。
