Blood Service, Belgian Red Cross, Namur, Belgium.
Laboratoire de Recherche sur les Produits Sanguins, Recherche et Développement Produits, Transfusion Interrégionale CRS, Epalinges, Switzerland.
Vox Sang. 2021 Jan;116(1):53-59. doi: 10.1111/vox.12979. Epub 2020 Aug 14.
The antioxidant power measurement can be useful to validate the execution of the pathogen inactivation treatment of platelet concentrates. The aim of this study is to evaluate the technology on different blood preparations including INTERCEPT and Mirasol treatments that are in routine use in Belgium and Luxemburg.
The antioxidant power measurement was tested on 78 apheresis platelet concentrates and 54 pools of buffy-coats-derived platelet concentrates before and after INTERCEPT treatment. In addition, 100 Reveos platelet pools were tested before and after Mirasol treatment. The antioxidant power was quantified electrochemically using disposable devices and was expressed as equivalent ascorbic acid concentration.
Mean results for apheresis platelet concentrates were of 90 ± 14 and 35 ± 10 µmol/l eq. ascorbic acid before and after INTERCEPT treatment, respectively. The mean results for pools of buffy-coats-derived platelet concentrates were of 81 ± 10 and 29 ± 4 eq. µmol/l ascorbic acid before and after INTERCEPT treatment, respectively. For buffy-coats-derived platelet concentrates treated by Mirasol technology, the mean results were of 98 ± 11 and 32 ± 10 µmol/l eq. ascorbic acid before and after illumination, respectively.
The antioxidant power significantly decreases with pathogen inactivation treatments for platelet concentrates treated by INTERCEPT or Mirasol technologies.
抗氧化能力的测量可用于验证血小板浓缩物的病原体灭活处理的执行情况。本研究的目的是评估不同血液制剂的技术，包括在比利时和卢森堡常规使用的 INTERCEPT 和 Mirasol 处理。
在进行 INTERCEPT 处理之前和之后，对 78 份单采血小板浓缩物和 54 份浓缩的富含血小板的白细胞池进行抗氧化能力测量。此外，对 100 个 Reveos 血小板池在进行 Mirasol 处理之前和之后进行了测试。抗氧化能力通过使用一次性设备进行电化学定量，并以当量抗坏血酸浓度表示。
单采血小板浓缩物的平均结果分别为 INTERCEPT 处理前后的 90 ± 14 和 35 ± 10 µmol/l 当量抗坏血酸。富含血小板的白细胞池的平均结果分别为 INTERCEPT 处理前后的 81 ± 10 和 29 ± 4 µmol/l 当量抗坏血酸。对于使用 Mirasol 技术处理的富含血小板的白细胞池，平均结果分别为光照前后的 98 ± 11 和 32 ± 10 µmol/l 当量抗坏血酸。
使用 INTERCEPT 或 Mirasol 技术处理的血小板浓缩物的病原体灭活处理会显著降低其抗氧化能力。
