Perez-Pujol S, Tonda R, Lozano M, Fuste B, Lopez-Vilchez I, Galan A M, Li J, Goodrich R, Escolar G
Hemotherapy-Hemostasis Service, CDB, Hospital Clinic, IDIBAPS, University of Barcelona, Spain.
Transfusion. 2005 Jun;45(6):911-9. doi: 10.1111/j.1537-2995.2005.04350.x.
Several strategies are being developed to reduce the risk of pathogen transmission associated with platelet (PLT) transfusion.
The impact of a new technology for pathogen reduction based on riboflavin plus illumination (Mirasol PRT, Navigant Biotechnologies, Inc.) at 6.2 and 12.3 J per mL on functional and biochemical characteristics of PLTs was evaluated. PLT concentrates (PCs) obtained by apheresis were treated with Mirasol PRT and stored at 22 degrees C. Modifications in major PLT glycoproteins (GPIbalpha, GPIV, and GPIIb-IIIa), adhesive ligands (von Willebrand factor [VWF], fibrinogen [Fg], and fibronectin), activation antigens (P-selectin and LIMP), and apoptotic markers (annexin V binding and factor [F]Va) were analyzed by flow cytometry. Adhesive and cohesive PLT functions were evaluated with well-established perfusion models. Studies were performed on the preparation day (Day 0) and during PCs storage (Days 3 and 5).
Levels of glycoproteins remained stable during storage in PCs treated with 6.2 J per mL pathogen reduction technology (PRT) and similar to those observed in nontreated PCs. When 12.3 J per mL PRT was applied, however, levels of GPIbalpha moderately decreased on Days 3 and 5. VWF, Fg, and FVa were not modified in their expression levels, either by treatment or by storage period. Fibronectin appeared more elevated in all PRT samples. A progressive increase in P-selectin and LIMP expression and in annexin V binding was observed during storage of PRT-treated PCs. Functional studies indicated that 6.2 J per mL Mirasol PRT-treated PLTs preserved adhesive and cohesive functions to levels compatible with those observed in the respective control PCs.
PLT function was well preserved in PCs treated with 6.2 J per mL Mirasol PRT and stored for 5 days.
目前正在研发多种策略以降低与血小板（PLT）输血相关的病原体传播风险。
评估了一种基于核黄素加光照的新型病原体灭活技术（Mirasol PRT，Navigant生物技术公司），其剂量为每毫升6.2焦耳和12.3焦耳，对PLT功能和生化特性的影响。通过单采获得的PLT浓缩物（PCs）用Mirasol PRT处理，并在22℃下储存。通过流式细胞术分析主要PLT糖蛋白（GPIbalpha、GPIV和GPIIb - IIIa）、黏附配体（血管性血友病因子[VWF]、纤维蛋白原[Fg]和纤连蛋白）、活化抗原（P - 选择素和LIMP）以及凋亡标志物（膜联蛋白V结合和因子[F]Va）的变化。采用成熟的灌注模型评估PLT的黏附与聚集功能。研究在制备当天（第0天）以及PCs储存期间（第3天和第5天）进行。
在用每毫升6.2焦耳病原体灭活技术（PRT）处理的PCs储存期间，糖蛋白水平保持稳定，与未处理的PCs中观察到的水平相似。然而，当应用每毫升12.3焦耳的PRT时，GPIbalpha水平在第3天和第5天适度下降。VWF、Fg和FVa的表达水平在处理或储存期间均未改变。在所有PRT样本中，纤连蛋白水平似乎更高。在PRT处理的PCs储存期间，观察到P - 选择素和LIMP表达以及膜联蛋白V结合逐渐增加。功能研究表明，用每毫升6.2焦耳Mirasol PRT处理的PLT的黏附与聚集功能保持在与相应对照PCs中观察到的水平相当。
用每毫升6.2焦耳Mirasol PRT处理并储存5天的PCs中，PLT功能得到良好保留。
