Department of Animal Physiology, Biochemistry and Biostructure, Poznań University of Life Sciences, 60-637, Poznań, Poland.
Department of Hepatology and Gastroenterology, Charité-University Medicine Berlin, 13353, Berlin, Germany; Department of Internal Medicine-Gastroenterology, Park-Klinik Weissensee, 13086, Berlin, Germany.
Arch Biochem Biophys. 2020 Oct 15;692:108536. doi: 10.1016/j.abb.2020.108536. Epub 2020 Aug 13.
Adropin is a peptide hormone encoded by Energy Homeostasis Associated (Enho) gene. Adropin modulates glucose and lipid metabolism, and adiposity. Recently, we found that adropin suppresses differentiation of rodent white preadipocytes into mature fat cells. By contrast, the role of adropin in controlling brown adipogenesis is largely unknown. Therefore, in the present study we evaluated the effects of adropin on proliferation and differentiation of adipocyte precursor cells in rats. Brown adipocyte precursor cells were isolated from male Wistar rats. Cell replication was measured by BrdU incorporation. Gene expression was studied using real time PCR. Protein phosphorylation and production was assessed by Western blot. Lipid accumulation was evaluated by Oil Red O staining. Colorimetric kits were used to evaluate glycerol and free fatty acids release. We report here that adropin stimulates proliferation of brown preadipocytes. Moreover, in brown preadipocytes, adropin suppresses mRNA expression of adipogenic genes (C/ebpα, C/ebpβ, Pgc1α, Pparγ and Prdm16) during differentiation process. In addition, adropin suppresses UCP1 protein production in brown adipocytes. Finally, adropin reduces intracellular lipid content in brown adipocytes. These results indicate that adropin stimulates proliferation of brown preadipocytes and suppresses their differentiation into mature adipocytes.
adiponectin 是一种由能量平衡相关 (Enho) 基因编码的肽激素。 adiponectin 调节葡萄糖和脂质代谢以及肥胖。最近，我们发现 adiponectin 抑制啮齿动物白色前体脂肪细胞分化为成熟脂肪细胞。相比之下，adiponectin 在控制棕色脂肪生成中的作用在很大程度上尚不清楚。因此，本研究评估了 adiponectin 对大鼠脂肪前体细胞增殖和分化的影响。棕色脂肪前体细胞从雄性 Wistar 大鼠中分离出来。通过 BrdU 掺入测量细胞复制。使用实时 PCR 研究基因表达。通过 Western blot 评估蛋白质磷酸化和产生。通过油红 O 染色评估脂质积累。使用比色试剂盒评估甘油和游离脂肪酸的释放。我们在这里报告，adiponectin 刺激棕色前体脂肪细胞的增殖。此外，在棕色前体脂肪细胞中，adiponectin 在分化过程中抑制脂肪生成基因 (C/ebpα、C/ebpβ、Pgc1α、Pparγ 和 Prdm16) 的 mRNA 表达。此外，adiponectin 抑制棕色脂肪细胞中 UCP1 蛋白的产生。最后，adiponectin 减少棕色脂肪细胞内的细胞内脂质含量。这些结果表明 adiponectin 刺激棕色前体脂肪细胞的增殖，并抑制其分化为成熟脂肪细胞。
