School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
Int J Biol Macromol. 2020 Dec 1;164:2204-2214. doi: 10.1016/j.ijbiomac.2020.08.093. Epub 2020 Aug 13.
Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity have exhibited great potential in clinical application in drug delivery and wound healing. In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS). The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio. The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus. After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed. Moreover, the excellent antibacterial activity of the composite hydrogel was also demonstrated in vitro. These results support the bioactive composite hydrogel can be employed as a promising injectable scaffold for promoting wound regeneration and drug delivery.
具有持续药物释放能力和抗菌活性的生物聚合物水凝胶在药物输送和伤口愈合的临床应用中显示出巨大的潜力。在这项研究中，通过将载药的结冷胶微球（GMs）嵌入双交联水凝胶中，开发出了一种新型的复合伤口敷料水凝胶，该水凝胶是通过氧化结冷胶（OG）（通过钙离子预交联）和羧甲基壳聚糖（CMCS）的席夫碱交联构建的。通过改变 CMCS/OG（w/w）与固定 OG/钙（w/w）比的比值，优化了空白水凝胶的凝胶时间、溶胀指数、降解率和机械性能。当 CMCS/OG 为 16/7（w/w）时，水凝胶具有最佳的整体性能，凝胶时间为 139 s，溶胀平衡后溶胀指数保持在 30 以上，第 7 天降解率为 100.5%，压缩模量为 8.8 KPa。负载货物的 GMs 嵌入后，空白水凝胶的上述性能得到了改善，观察到货物（抗菌药物盐酸四环素和磺胺嘧啶银）的持续释放。此外，复合水凝胶在体外也表现出优异的抗菌活性。这些结果支持生物活性复合水凝胶可用作一种有前途的可注射支架，用于促进伤口再生和药物输送。
