Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.
Environ Pollut. 2020 Nov;266(Pt 2):115310. doi: 10.1016/j.envpol.2020.115310. Epub 2020 Aug 1.
Spirotetramat, a member of tetronic and tetramic acid derivatives, is a unique insecticide and acaricide. Although the effect on zebrafish embryos lipid biosynthesis of spirotetramat has been characterized, the energy metabolism and toxic effect mechanism warrant further investigation. To investigate the toxic mechanism of spirotetramat on energy metabolism, zebrafish embryos were exposed to 100, 500 and 1000 µg/L of spirotetramat for 4 days. Untargeted metabolomics showed the synthesis and degradation of ketone pathway metabolites (R)-3-Hydroxybutyric acid and Acetoacetate significantly decreased, as well as increasing the abundance of Anti-Acetyl Coenzyme A Carboxylase protein (ACC1). Down-regulation of the genes related to ß-oxidation and the tricarboxylic acid cycle in the embryos show decreased energy metabolism. Carnitine palmitoyltransferase 1 (CPT- I) significantly decreased while citrate synthase (CS) significantly increased. Additionally, mitochondrial lesions in embryos were found using electron microscopy. Our study provides novel and robust perspectives, which show that spirotetramat treatment in embryos leads to metabolic disturbances that adversely affect cellular energy homeostasis.
螺虫乙酯，四氢呋喃和四氢噻唑酸衍生物的一员，是一种独特的杀虫剂和杀螨剂。虽然已经对螺虫乙酯对斑马鱼胚胎脂质生物合成的影响进行了描述，但能量代谢和毒性作用机制仍需要进一步研究。为了研究螺虫乙酯对能量代谢的毒性作用机制，将斑马鱼胚胎暴露于 100、500 和 1000μg/L 的螺虫乙酯中 4 天。非靶向代谢组学显示酮途径代谢物（R）-3-羟基丁酸和乙酰乙酸的合成和降解显著减少，同时增加了抗乙酰辅酶 A 羧化酶蛋白（ACC1）的丰度。胚胎中与β-氧化和三羧酸循环相关的基因下调表明能量代谢减少。肉碱棕榈酰转移酶 1（CPT-I）显著降低，而柠檬酸合酶（CS）显著增加。此外，还用电镜观察到胚胎中的线粒体损伤。我们的研究提供了新的和有力的观点，表明螺虫乙酯处理胚胎会导致代谢紊乱，从而对细胞能量稳态产生不利影响。
