Carlos Chagas Filho Biophysics Institute, Federal University of Rio De Janeiro , Rio De Janeiro, Brazil.
Department of Surgery, Clementino Fraga Filho Universitary Hospital, Federal University of Rio De Janeiro , Rio De Janeiro, Brazil.
Organogenesis. 2020 Jul 2;16(3):95-112. doi: 10.1080/15476278.2020.1801273. Epub 2020 Aug 15.
Acellular liver scaffolds (ALS) have arisen as potential candidates for transplantation. Until now, all reports involving ALS transplantation failed in surgical method descriptions and do not offer support to scientists to reproduce the procedures used in experimental microsurgery to make the results comparable to literature. To overcome the lack of detail information, we described surgical steps details to perform heterotopic and partial orthotopic surgical models to promote ALS transplantation. After preservation and vessel cannulation steps, the liver grafts were decellularized. In addition, blood perfusion tests were performed to obtain a successful anticoagulation treatment prior transplantation. Then, methods of partial liver resection, combination of hand-suture and cuff techniques to complete end-to-end anastomosis between the scaffold and the recipient animal were performed. These procedures which take 30-60 min and were efficient to allow acellular liver scaffold viability and recellularization of different types of cell post-surgery. In conclusion, our methods are practical and simple promising approach that provides the opportunity to investigate ways to achieve sufficient liver function post-transplantation .
去细胞肝脏支架 (ALS) 已成为移植的潜在候选者。到目前为止，所有涉及 ALS 移植的报告在手术方法描述方面都失败了，并且不能为科学家提供支持，以重现用于实验性微创手术的程序，使结果与文献相比具有可比性。为了克服缺乏详细信息的问题，我们详细描述了进行异位和部分原位手术模型的手术步骤，以促进 ALS 移植。在保存和血管插管步骤之后，对肝移植物进行去细胞化处理。此外，进行血液灌注测试以在移植前获得成功的抗凝治疗。然后，进行部分肝切除术、手工缝合和套管技术相结合的方法，以完成支架和受体动物之间的端端吻合。这些程序耗时 30-60 分钟，效率高，可以使去细胞肝脏支架在手术后具有活力，并使不同类型的细胞再细胞化。总之，我们的方法是一种实用且简单的有前途的方法，为研究如何在移植后获得足够的肝功能提供了机会。
