Department of Comparative Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, 11211, Saudi Arabia.
Organ Transplantation Center, King Faisal Specialist Hospital and Research Centre, Riyadh, 11211, Saudi Arabia.
J Biomed Mater Res A. 2019 Feb;107(2):392-402. doi: 10.1002/jbm.a.36551. Epub 2018 Dec 3.
Decellularized liver scaffolds based liver engineering is a promising approach toward developing functional liver surrogates. However, a major obstacle to long-term transplantation is the hemocompatibility of the bioengineered liver surrogates. One approach to improve the hemocompatibility of engineered liver surrogates is re-endothelialization. In the current study, immortalized endothelial cells were perfused for re-endothelialization of decellularized rat liver scaffolds. When compared to the media-based perfusion approach, gelatin hydrogels-based perfusion significantly increased the number of cells that were retained in the decellularized liver scaffolds and the vascular lumen coverage ratio. Endothelial cells were lining along the vasculatures of the decellularized liver scaffolds and actively proliferating. Re-endothelialization improved the blood retention ability of the liver scaffold vasculatures. Doppler ultrasound detected active blood flows within the re-endothelialized liver scaffold transplants 8 days post-transplantation. Our results strengthened the feasibility of developing bioengineered liver surrogates utilizing decellularized liver scaffolds. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 392-402, 2019.
基于去细胞化肝脏支架的肝脏工程是开发功能性肝脏替代物的一种很有前途的方法。然而，长期移植的一个主要障碍是生物工程肝脏替代物的血液相容性。提高工程化肝脏替代物血液相容性的一种方法是再内皮化。在本研究中，使用永生化内皮细胞对去细胞化大鼠肝脏支架进行再内皮化。与基于培养基的灌注方法相比，明胶水凝胶基灌注显著增加了保留在去细胞化肝脏支架中的细胞数量和血管腔覆盖率。内皮细胞沿着去细胞化肝脏支架的血管排列，并积极增殖。再内皮化提高了肝支架血管的血液保留能力。多普勒超声在移植后 8 天检测到再内皮化肝支架移植物内的活跃血流。我们的结果加强了利用去细胞化肝脏支架开发生物工程肝脏替代物的可行性。 © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 392-402, 2019.
