Research Genetic Cancer Centre International GmbH, Zug 6300, Switzerland.
Research Genetic Cancer Centre S.A., Florina 53100, Greece.
Biomark Med. 2020 Oct;14(14):1317-1327. doi: 10.2217/bmm-2020-0372. Epub 2020 Aug 17.
Hormone-refractory prostate carcinoma has a different cell surface protein profile than hormone-sensitive prostate carcinoma, which provides migration ability and interactions with organs/tissues. Detection and association of these proteins with lymph node metastasis via lymphadenectomy might be beneficial for patients. Gene expression analysis in hormone-refractory and hormone-sensitive commercial cancer cell lines was performed and, after co-cultivation with osteoblasts or endothelial cells, knockdown experiments followed to validate potential biomarkers. "", their knockdown altered the expression of key regulators of endothelial-mesenchymal transition, invasion, motility and migration. In primary prostate tumors, these genes could be an indicator for future metastasis into lymph nodes.
激素难治性前列腺癌的细胞表面蛋白谱与激素敏感性前列腺癌不同，这为其提供了迁移能力和与器官/组织的相互作用。通过淋巴结切除术检测和关联这些蛋白与淋巴结转移可能对患者有益。对激素难治性和激素敏感性商业癌细胞系进行基因表达分析，然后进行共培养与成骨细胞或内皮细胞，进行敲低实验以验证潜在的生物标志物。结果表明，在共培养后，这些基因的敲低改变了关键的血管内皮-间质转化、侵袭、运动和迁移调节因子的表达。在原发性前列腺肿瘤中，这些基因可能是未来淋巴结转移的指标。
