Department of Biomedical Sciences, National Chung Cheng University, Chia-Yi, 621, Taiwan.
Division of Allergy, Immunology and Rheumatology, Department of Medicine, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Chia-Yi, 62247, Taiwan; School of Medicine, Tzu Chi University, Hualien, 970, Taiwan.
Biochem Biophys Res Commun. 2020 Oct 15;531(2):236-241. doi: 10.1016/j.bbrc.2020.07.030. Epub 2020 Aug 13.
Phostensin (PTS) encoded by KIAA1949 is a protein phosphatase 1 (PP1)-binding protein. In order to explore the cellular functions of PTS, we have searched PTS-binding proteins by using co-immunoprecipitation in combination with shotgun proteomics. Here, we report two novel PTS-binding proteins, Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. PTS associated with EHD proteins was also observed in GST pull-down assays. Immunofluorescence microscopy demonstrated that the complex was co-localized at the endocytic vesicles. EHD proteins have been known to play a critical role in regulation of endocytic transport. Overexpression of PTS-β can attenuate the endocytic trafficking of transferrin.
Phostensin (PTS) 由 KIAA1949 编码，是一种蛋白磷酸酶 1 (PP1) 结合蛋白。为了探索 PTS 的细胞功能，我们通过免疫共沉淀结合shotgun 蛋白质组学方法搜索 PTS 结合蛋白。在这里，我们报告了两个新的 PTS 结合蛋白，Eps15 同源结构域蛋白 1 (EHD1) 和 EHD4。GST 下拉实验也观察到 PTS 与 EHD 蛋白的结合。免疫荧光显微镜显示，该复合物在胞内体中共定位。EHD 蛋白在调节胞吞运输中起着关键作用。PTS-β 的过表达可以减弱转铁蛋白的胞吞运输。
