Department of Internal Medicine and Nephrology, Cairo University, Cairo, Egypt.
Department of Biochemistry, Cairo University, Cairo, Egypt.
Saudi J Kidney Dis Transpl. 2020 Jul-Aug;31(4):787-795. doi: 10.4103/1319-2442.292312.
Heat-shock proteins (HSPs) are a group of proteins that function to protect cells and tissues against different types of damage. The aim of this work was to study the relationship between the genetic variation in HSP70 genes and the risk for development of nephropathy in Egyptian patients with Type 2 diabetes mellitus (DM). This study was carried out on 90 patients divided into three groups: 30 patients of Type 2 DM with nephropathy (Group I), 30 patients of Type 2 DM without nephropathy (Group II) with duration of diabetes > 10 years in both patient groups, and 30 healthy persons, who served as controls (Group III). All the studied patients were submitted to full history taking, complete clinical examination, and laboratory investigations including fasting blood glucose, glycated hemoglobin, renal function tests, and urinary albumin- to-creatinine ratio. HSP70-1 -110 AC, +190 G/C, HSP70-2 +1267 A/G, and shock protein70- hom +2437 T/C gene polymorphism were determined using the polymerase chain reaction- restriction fragment length polymorphism technique (PCR-RFLP). The results of the present study showed a highly statistically significant difference between Group I and Group II regarding family history, systolic and diastolic blood pressure, and duration of diabetes. There was a significant difference in the distribution of C allele of HSP70-1 -110A/C and +190 G/C and G allele of HSP70-2+1267A/G with more frequent detection in nephropathy group versus other groups, while there was no significant difference in genotype and allele distributions among the three studied groups for the HSP70-hom. It can be concluded that the C allele distribution of (HSP70-1 -110 A/C and HSP70+190 C/G) and the G allele distribution of HSP70-2 +1267A/G are associated with the susceptibility to renal complications in Egyptian patients with Type 2 DM.
热休克蛋白（HSPs）是一组能够保护细胞和组织免受各种损伤的蛋白质。本研究旨在探讨 HSP70 基因的遗传变异与埃及 2 型糖尿病患者肾病发病风险的关系。该研究共纳入 90 例患者，分为三组：30 例 2 型糖尿病合并肾病（I 组）、30 例 2 型糖尿病无肾病（II 组）且糖尿病病程>10 年，以及 30 例健康人（III 组）。所有研究对象均接受详细病史询问、全面临床检查和实验室检查，包括空腹血糖、糖化血红蛋白、肾功能检查和尿白蛋白与肌酐比值。采用聚合酶链反应-限制性片段长度多态性技术（PCR-RFLP）检测 HSP70-1-110AC、+190GC、HSP70-2+1267AG 和 shockprotein70-hom+2437TC 基因多态性。本研究结果显示，I 组和 II 组在家族史、收缩压和舒张压以及糖尿病病程方面存在高度统计学差异。HSP70-1-110AC 和+190GC 的 C 等位基因以及 HSP70-2+1267AG 的 G 等位基因在肾病组中的分布存在显著差异，而 HSP70-hom 的基因型和等位基因分布在三组间无显著差异。综上所述，HSP70-1-110AC 和 HSP70+190GC 的 C 等位基因以及 HSP70-2+1267AG 的 G 等位基因分布与埃及 2 型糖尿病患者发生肾脏并发症的易感性相关。
