Kartal Seyfi, Kip Gülay, Küçük Ayşegül, Aşçı Seyhan Sümeyra, Erdem Özlem, Arslan Mustafa, Kavutçu Mustafa
Health Science University, Kanuni Training and Research Hospital, Department of Anaesthesiology and Reanimation, Trabzon, Turkey.
Gazi University, School of Medicine, Department of Anaesthesiology and Reanimation, Ankara, Turkey.
Drug Des Devel Ther. 2020 Jul 23;14:2937-2943. doi: 10.2147/DDDT.S258921. eCollection 2020.
The objective of this research was to evaluate the oxidative and histopathological effects of dexmedetomidine and ketamine on the pulmonary contusion model resulting from blunt chest trauma.
Rats were randomly assigned to 5 equal groups (n=6): control group (Group C), pulmonary contusion group (Group PC), PC-dexmedetomidine group (Group PC-D), PC-ketamine group (Group PC-K), and PC-dexmedetomidine + ketamine (Group PC-D+K). The PC was performed by dropping a weight of 500 g (2.45 Joules) from a height of 50 cm. In Group PC-D, after chest trauma, dexmedetomidine (100 µg/kg) was administered intraperitoneally. In Group PC-K, after chest trauma, ketamine (100 mg/kg) was administered intraperitoneally. In Group PC-D+K, dexmedetomidine and ketamine were administered together. At the end of the 6th hour, rats were sacrificed. Malondialdehyde (MDA) level, superoxide dismutase (SOD) enzyme activities, neutrophil infiltration/aggregation, and thickness of the alveolar wall were evaluated.
MDA levels were significantly higher in Group PC than Groups C, PC-D, and PC-D+K. SOD enzyme activity was significantly higher in Group PC than Groups C, PC-D, and PC-D+K. In addition, neutrophil infiltration/aggregation and total pulmonary injury scores were significantly higher in Group PC than in other groups, and the thickness of the alveolar wall was significantly higher in Group PC compared to Groups C, PC-D, and PC-D+K. MDA level, SOD enzyme activities, neutrophil infiltration/aggregation, and thickness of alveolar wall were similar in PC-D and PC-D+K groups.
Dexmedetomidine and dexmedetomidine+ketamine have protective effects on blunt chest trauma but no protective effect was observed when ketamine was administered alone. We concluded that the administration of dexmedetomidine and ketamine after contusion is beneficial against pulmonary injury in rats.
本研究的目的是评估右美托咪定和氯胺酮对钝性胸部创伤所致肺挫伤模型的氧化和组织病理学影响。
将大鼠随机分为5个相等的组（n = 6）：对照组（C组）、肺挫伤组（PC组）、PC-右美托咪定组（PC-D组）、PC-氯胺酮组（PC-K组）和PC-右美托咪定+氯胺酮组（PC-D+K组）。通过从50 cm高度扔下500 g（2.45焦耳）的重物来造成肺挫伤。在PC-D组中，胸部创伤后，腹腔注射右美托咪定（100 μg/kg）。在PC-K组中，胸部创伤后，腹腔注射氯胺酮（100 mg/kg）。在PC-D+K组中，同时给予右美托咪定和氯胺酮。在第6小时末，处死大鼠。评估丙二醛（MDA）水平、超氧化物歧化酶（SOD）酶活性、中性粒细胞浸润/聚集以及肺泡壁厚度。
PC组的MDA水平显著高于C组、PC-D组和PC-D+K组。PC组的SOD酶活性显著高于C组、PC-D组和PC-D+K组。此外，PC组的中性粒细胞浸润/聚集和总肺损伤评分显著高于其他组，且PC组的肺泡壁厚度显著高于C组、PC-D组和PC-D+K组。PC-D组和PC-D+K组的MDA水平、SOD酶活性、中性粒细胞浸润/聚集以及肺泡壁厚度相似。
右美托咪定和右美托咪定+氯胺酮对钝性胸部创伤有保护作用，但单独给予氯胺酮时未观察到保护作用。我们得出结论，挫伤后给予右美托咪定和氯胺酮对大鼠肺损伤有益。
