Kucuk A, Yaylak F, Cavunt-Bayraktar A, Tosun M, Arslan M, Comu F M, Kavutcu M
Bratisl Lek Listy. 2014;115(11):680-4. doi: 10.4149/bll_2014_132.
The aim of this study was to evaluate the effect of dexmedetomidine (100 µg/kg-ip) on liver ischemia and reperfusion (I/R) in rats.
Twenty-four Wistar Albino rats were separated into three groups as control (C), ischemia-reperfusion injury (I/R) and dexmedetomidine group (I/R-D). Ischemia was induced with portal clampage for 45 minutes and reperfusion period was 45 minutes after declampage. Group I/R-D was received dexmedetomidine 100 µg/kg i.p. 30 min before portal clampage. Thiobarbutiric Acid-Reactive Substances (TBARS), glutathioneS-transferase (GST), superoxide dismutase (SOD), Catalase (CAT), and Paraoxonase 1 (PON-1) were investigated in blood samples. Also HSP60 and p53-positive hepatocytes were counted under ImageJ image analysis program.
All parameters, except GST levels, were significant between the groups (p < 0.05). Although HSP60 expression was significantly increased between I/R, I/R-D and C groups there were no significant differences between I/R-D and C (p = 0.443). On the other hand, p53 expression was also significantly increased between I/R, I/R-D and C groups At the same time, there were no significant differences between I/R-D and C groups (p = 0.354).
All the results suggest that dexmedetomidine has beneficial effects on liver ischemia/reperfusion stress (Tab. 1, Fig. 2, Ref. 49).
本研究旨在评估右美托咪定（100μg/kg腹腔注射）对大鼠肝脏缺血再灌注（I/R）的影响。
将24只Wistar白化大鼠分为三组，即对照组（C）、缺血再灌注损伤组（I/R）和右美托咪定组（I/R-D）。通过门静脉夹闭45分钟诱导缺血，夹闭解除后再灌注45分钟。I/R-D组在门静脉夹闭前30分钟腹腔注射100μg/kg右美托咪定。检测血样中的硫代巴比妥酸反应物质（TBARS）、谷胱甘肽S-转移酶（GST）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和对氧磷酶1（PON-1）。同时，在ImageJ图像分析程序下对HSP60和p53阳性肝细胞进行计数。
除GST水平外，所有参数在各组间均有显著差异（p<0.05）。虽然I/R组、I/R-D组和C组之间HSP60表达显著增加，但I/R-D组和C组之间无显著差异（p=0.443）。另一方面，I/R组、I/R-D组和C组之间p53表达也显著增加，同时，I/R-D组和C组之间无显著差异（p=0.354）。
所有结果表明，右美托咪定对肝脏缺血/再灌注应激具有有益作用（表1，图2，参考文献49）。
