Kirby J A, Reader J A, Parfett G J, Pepper J R
Department of Immunology, St George's Hospital Medical School, London, UK.
Clin Exp Immunol. 1988 Jan;71(1):113-9.
Limiting dilution analysis was used to measure the frequency of PVG-reactive cytotoxic T lymphocytes (CTL) within the peripheral blood mononuclear cell population of Lewis rats before heterotopic transplantation of PVG rat derived cardiac tissue, and in both the blood and graft-infiltrating cell populations at daily time points afterwards. Before surgery, the frequency of PVG-reactive cells within the blood was between 1/31,700 and 1/50,300; however, this value increased rapidly on day 4 after transplantation to reach values of up to 1/1,100 by day 7. The frequency of these cells was first measurable in the graft-infiltrate on day 2 and also showed a rapid increase 4 days after surgery; peak values up to 1/4,800 were recorded on day 5. This time corresponded with that of functional cardiac rejection and maximum infiltration of the graft by mononuclear cells. The similar kinetic changes and absolute values recorded for the frequency of donor-reactive CTL within the blood and graft-derived cell populations was indicative of a rapid bi-directional passage of cells between these pools and provided no evidence for specific sequestration of CTL by the graft. Cells purified from the graft on post-operative day 5 mediated an immediate specific cytotoxicity towards PVG target cells (44% lysis during a 4 h assay at an effector:target ratio of 100:1) which was of a higher activity than would be predicted on the basis of an effector population containing only 1/4,800 PVG-reactive CTL. This finding implies that other mononuclear cell types than CTL present within the graft-infiltrating population were capable of mediating target cells lysis.
在移植PVG大鼠来源的心脏组织前，采用有限稀释分析法测定Lewis大鼠外周血单个核细胞群体中对PVG产生反应的细胞毒性T淋巴细胞（CTL）的频率，并在术后每天测定血液和移植物浸润细胞群体中的该频率。术前，血液中对PVG产生反应的细胞频率在1/31,700至1/50,300之间；然而，移植后第4天该值迅速增加，到第7天达到高达1/1,100的值。这些细胞的频率在术后第2天首次在移植物浸润物中可检测到，并且在术后4天也显示出快速增加；第5天记录到高达1/4,800的峰值。这个时间与功能性心脏排斥反应以及移植物被单核细胞最大程度浸润的时间一致。血液和移植物来源的细胞群体中供体反应性CTL频率记录的相似动力学变化和绝对值表明这些池之间细胞的快速双向传递，并且没有提供CTL被移植物特异性隔离的证据。术后第5天从移植物中纯化的细胞对PVG靶细胞介导了即时特异性细胞毒性（在效应细胞与靶细胞比例为100:1的4小时检测中44%的裂解率），其活性高于仅含有1/4,800 PVG反应性CTL的效应细胞群体所预测的活性。这一发现意味着移植物浸润群体中除CTL之外的其他单核细胞类型能够介导靶细胞裂解。
