Oluwole S, Satake K, Kuromoto N, Fawwaz R, Hardy M A
Transplantation. 1983 Nov;36(5):558-64. doi: 10.1097/00007890-198311000-00016.
The kinetics of lymphocyte recirculation in normal and allografted rats with acute cardiac rejection was studied with indium-111 (In-111) labeled splenic lymphocytes in two groups of rats. Group 1 consisted of subgroups of normal Lewis rats infused with In-111 labeled unsensitized syngeneic cells (group 1a); ACI-sensitized syngeneic cells (group 1b); and ACI spleen cells (group 1c). Four rats from each subgroup were killed at 3, 6, 18, and 24 hr after cell infusion for blood, spleen, mesenteric lymph node (MLN), thymus, bone marrow (BM), liver, kidney, muscle, and heart scintillation counts. Group 2 consisted of Lewis recipients of ACI cardiac allografts infused with normal or with ACI-sensitized syngeneic splenic cells. Four rats from each subgroup were killed daily until rejection (day 7) for isotope counts of various organs. In ungrafted rats (group I), splenic accumulation of unsensitized syngeneic cells fell from 50% of the total injected dose/g tissue at 3 hr to 28% at 24 hr, whereas it rose from 12% at 3 hr to 39% at 24 hr in MLN. In contrast, the sensitized syngeneic and allogeneic cells homed preferentially to the spleen with insignificant accumulation in the MLN throughout the experiment. The BM and liver showed moderate accumulation while the thymus and nonlymphoid organs had low concentrations of labeled cells at all times. Splenic accumulation of unsensitized syngeneic cells in allografted rats (group II) showed a steep rise from day 1, reaching a peak at day 3, followed by a plateau--but sensitized cells demonstrated a peak on day 4 followed by a sharp decline until rejection. Accumulation of unsensitized cells in the MLN was significantly higher (P less than 0.001) than that of sensitized cells throughout the study. There was a significant fall (P less than 0.001) in radioactivity of BM, thymus, liver, and nonlymphoid organs from days 1-7, and the cardiac allograft demonstrated a reciprocal sharp rise in radioactivity. There was a significant early accumulation (P less than 0.001) of sensitized cells compared with unsensitized cells in the cardiac allograft on day 1. This study shows that In-111 labeled donor cells bearing surface antigen different from that of the recipient were sequestered from the circulating pool and immobilized in the spleen, but labeled donor cells with similar surface antigen to that of the recipient were recruited into the lymph node lymphocyte recirculating pool. It further demonstrates the difference in migration patterns of normal and sensitized syngeneic cells during acute allograft rejection.
用铟 - 111（In - 111）标记的脾淋巴细胞研究了正常大鼠和发生急性心脏排斥反应的同种异体移植大鼠体内淋巴细胞再循环的动力学，分为两组大鼠。第1组由正常Lewis大鼠亚组组成，分别注入In - 111标记的未致敏同基因细胞（1a组）、ACI致敏的同基因细胞（1b组）和ACI脾细胞（1c组）。每组4只大鼠在细胞注入后3、6、18和24小时处死，进行血液、脾脏、肠系膜淋巴结（MLN）、胸腺、骨髓（BM）、肝脏、肾脏、肌肉和心脏的闪烁计数。第2组由接受ACI心脏同种异体移植的Lewis受体组成，分别注入正常或ACI致敏的同基因脾细胞。每组4只大鼠每天处死，直至排斥反应发生（第7天），对各器官进行同位素计数。在未移植的大鼠（第I组）中，未致敏同基因细胞在脾脏中的蓄积量从注入后3小时的50%（每克组织注射总剂量）降至24小时的28%，而在MLN中则从3小时的12%升至24小时的39%。相反，致敏的同基因细胞和异基因细胞在整个实验过程中优先归巢至脾脏，在MLN中的蓄积不明显。BM和肝脏显示出中等程度的蓄积，而胸腺和非淋巴器官在所有时间点的标记细胞浓度都很低。同种异体移植大鼠（第II组）中未致敏同基因细胞的脾脏蓄积量从第1天开始急剧上升，在第3天达到峰值，随后趋于平稳——但致敏细胞在第4天达到峰值，随后急剧下降直至排斥反应发生。在整个研究过程中，MLN中未致敏细胞的蓄积量显著高于致敏细胞（P小于0.001）。从第1天到第7天，BM、胸腺、肝脏和非淋巴器官的放射性显著下降（P小于0.001），而心脏同种异体移植的放射性则相应急剧上升。在第1天，心脏同种异体移植中致敏细胞与未致敏细胞相比有显著的早期蓄积（P小于0.001）。本研究表明，表面抗原与受体不同的In - 111标记供体细胞从循环池中被隔离并固定在脾脏中，但表面抗原与受体相似的标记供体细胞被募集到淋巴结淋巴细胞再循环池中。它进一步证明了急性同种异体移植排斥反应期间正常和致敏同基因细胞迁移模式的差异。
