Shao Shuai, Wang Ming-Xing, Zhang Hong-Yin, Fan Lin, Han Rong-Xin, Shen Ying-Xin, Yan Ming-Ming, Zhao Da-Qing
Changchun University of Chinese Medicine, Changchun, Jilin 130117, China.
Jilin Provincial Science and Technology Innovation Center of Health Food of Chinese Medicine, Changchun University of Chinese Medicine, Changchun, Jilin, China.
Evid Based Complement Alternat Med. 2020 Jul 29;2020:4231340. doi: 10.1155/2020/4231340. eCollection 2020.
The glycoprotein from was obtained with alkali extraction and acid precipitation, purified with DEAE Sepharose Fast Flow and Superdex G-75 column. The molecular composition structure and antifatigue activities of glycoprotein were studied. SCGP's molecular weight was approximately 10 KDa, and it consisted of a carbohydrate component (52.94%) and protein component (47.06%). SCGP comprised mannose, galactoside, rhamnose, glucose, galactose, xylose, arabinose, and fucose, its molar ratio was 2.14 : 1.43 : 1.59 : 8.17 : 8.99 : 3.18 : 18.51 : 1, and it contained 16 kinds of amino acids. SCGP could obviously extend the swimming time in mice by increasing LDH, SOD level, GSH-Px activity, and liver glycogen and decreasing the contents of BUN and MDA. The antioxidant activity of SCGP is a potential mechanism of its antifatigue effect. In vitro antioxidant test showed that SCGP scavenged DPPH and OH radicals in a dose-dependent manner (IC was 0.91 mg/ml and 0.72 mg/ml).
通过碱提取和酸沉淀从[具体来源未提及]中获得糖蛋白，并用DEAE Sepharose Fast Flow和Superdex G - 75柱进行纯化。研究了糖蛋白的分子组成结构和抗疲劳活性。SCGP的分子量约为10 kDa，由碳水化合物成分（52.94%）和蛋白质成分（47.06%）组成。SCGP包含甘露糖、半乳糖苷、鼠李糖、葡萄糖、半乳糖、木糖、阿拉伯糖和岩藻糖，其摩尔比为2.14∶1.43∶1.59∶8.17∶8.99∶3.18∶18.51∶1，且含有16种氨基酸。SCGP可通过提高LDH、SOD水平、GSH - Px活性和肝糖原含量以及降低BUN和MDA含量，明显延长小鼠的游泳时间。SCGP的抗氧化活性是其抗疲劳作用的潜在机制。体外抗氧化试验表明，SCGP以剂量依赖性方式清除DPPH和OH自由基（IC分别为0.91 mg/ml和0.72 mg/ml）。
