Li Yu-Xian, Yang Zhong-Han, Lin Yin, Han Wei, Jia Shan-Shan, Yuan Ke
College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450008, P. R. China.
College of Chemical Industry and Environment Engineering, Jiaozuo University, Jiaozuo 454000, P. R. China.
Pharmacogn Mag. 2016 Jul-Sep;12(47):219-24. doi: 10.4103/0973-1296.186341.
The aim of this study is to determine the antifatigue active fraction from Abelmoschus esculentus. The in vivo antifatigue effects of ethanol extracts and polysaccharides from A. esculentus fruit have been determined. The polysaccharides of A. esculentus were determined as the best effective fractions of antifatigue effects.
About 360 Kunming male mice were randomly divided into nine subgroups: normal control subgroup, model subgroup, positive subgroup and the ethanol extracts of A. esculentus with high dose (3.2 g/kg) subgroup, medium dose (1.6 g/kg) subgroup and low dose (0.8 g/kg) subgroup, the polysaccharides of high dose (3.2 g/kg) subgroup, medium dose (1.6 g/kg) subgroup, and the low dose (0.8 g/kg) subgroup. The antifatigue effects of ethanol extracts and polysaccharides form A. esculentus were measured by comparing body weight, food intake, swimming time, liver glycogen, serum urea, blood lactic acid as well as visceral parameter in mice.
Compared with the model subgroup, other subgroups significantly prolonged swimming time, and high dose polysaccharides administration was the most effective (P < 0.01). High dose polysaccharides significantly increased liver glycogen, serum lactic acid, and serum urea (P < 0.01) in mice. In contrast with model group, the high dose polysaccharides administration could also significantly elevated the parameters of testicles and epididymis (P < 0.01). The study established that the ethanol extracts and polysaccharides of A. esculentus both have antifatigue effects.
The results demonstrated that both the ethanol extracts and polysaccharides of A. esculentus have antifatigue effects. The high dosage polysaccharides have significant antifatigue properties. The results will provide the basis for further development and utilization of this plant.
The high dosage polysaccharides have restoration ability on kidney yang deficiency mice.The high dosage polysaccharides have significant effects of relieving body fatigue of mice.The polysaccharide of Abelmoschus esculentus showed better antifatigue effects than the ethanol extracts. Abbreviations used: A. esculentus: Abelmoschus esculentus, BUN: Blood urine nitrogen, LD: Lactic Acid dehydrogenase, AE: Abelmoschus esculentus ethanol extracts, AP: Abelmoschus esculentus polysaccharides, LAC: Lactic acid content.
本研究旨在确定黄秋葵的抗疲劳活性成分。已测定黄秋葵果实乙醇提取物和多糖的体内抗疲劳作用。黄秋葵多糖被确定为抗疲劳效果最佳的有效成分。
将约360只昆明雄性小鼠随机分为9个亚组：正常对照组、模型亚组、阳性亚组以及黄秋葵乙醇提取物高剂量（3.2 g/kg）亚组、中剂量（1.6 g/kg）亚组、低剂量（0.8 g/kg）亚组，多糖高剂量（3.2 g/kg）亚组、中剂量（1.6 g/kg）亚组、低剂量（0.8 g/kg）亚组。通过比较小鼠的体重、食量、游泳时间、肝糖原、血清尿素、血乳酸以及脏器参数来测定黄秋葵乙醇提取物和多糖的抗疲劳作用。
与模型亚组相比，其他亚组均显著延长了游泳时间，且高剂量多糖给药效果最为显著（P < 0.01）。高剂量多糖显著增加了小鼠的肝糖原、血清乳酸和血清尿素（P < 0.01）。与模型组相比，高剂量多糖给药还可显著提高睾丸和附睾的参数（P < 0.01）。该研究证实黄秋葵的乙醇提取物和多糖均具有抗疲劳作用。
结果表明黄秋葵的乙醇提取物和多糖均具有抗疲劳作用。高剂量多糖具有显著的抗疲劳特性。研究结果将为该植物的进一步开发利用提供依据。
高剂量多糖对肾阳虚小鼠具有恢复能力。高剂量多糖对小鼠具有显著的缓解身体疲劳作用。黄秋葵多糖的抗疲劳效果优于乙醇提取物。缩写词：A. esculentus：黄秋葵，BUN：血尿素氮，LD：乳酸脱氢酶，AE：黄秋葵乙醇提取物，AP：黄秋葵多糖，LAC：乳酸含量。
