Faculty of Pharmacy, Institute of Health Sciences, Federal University of Pará, Belém, 66075-900 Pará, Brazil.
State University of Central-West, Department of Food Engineering (DEALI), Cedeteg Campus, Vila Carli, R. Simeão Varela de Sá, Guarapuava, 85040-080 Paraná, Brazil.
Oxid Med Cell Longev. 2020 Jul 30;2020:2497845. doi: 10.1155/2020/2497845. eCollection 2020.
, mushroom used for centuries by Asian peoples as food supplement, has been shown interesting biological activities, including over the Central Nervous System. Besides, these mushroom bioactive compounds present antioxidant and anti-inflammatory activities. On the side, binge drinking paradigm consists of ethanol exposure that reflects the usual consumption of adolescents, which elicits deleterious effects, determined by high ethanol consumption, in a short period. In this study, we investigated whether the Aqueous Extract of (AEGl) reduces the behavioral disorders induced by alcohol. Male ( = 30) and female Wistar rats ( = 40), seventy-two days old, were used for behavioral/biochemical and oral toxicity test, respectively. Animals were exposed to 5 binges (beginning at 35 days old) of ethanol (3 g/kg/day) or distilled water. Twenty-four hours after the last binge administration, animals received AEGl (100 mg/kg/day) or distilled water for three consecutive days. After treatment protocol, open field, elevated plus maze, forced swim, and step-down inhibitory avoidance tests were performed. Oxidative stress parameters were measured to evaluate the REDOX balance. Our results demonstrated that AEGl elicited the recovery of spontaneous horizontal exploration capacity, anxiogenic- and depressive-profile, as well as short-term memory damage induced by binge-ethanol exposure. The behavioral effects of the extract were associated to the reequilibrium of the animals' REDOX balance. Thus, AEGl, a medicinal mushroom, ameliorates behavioral alteration on a model of motor, cognitive and psychiatric-like disorders induced by binge drinking paradigm and emerges as a useful tool as a food supplement in the management of disorders of alcoholic origin.
，一种被亚洲人民用作食品补充剂已有数百年历史的蘑菇，已显示出有趣的生物活性，包括对中枢神经系统的作用。此外，这些蘑菇的生物活性化合物具有抗氧化和抗炎作用。另一方面， binge drinking 范式包括乙醇暴露，反映了青少年的通常消费习惯，在短时间内会产生高乙醇消耗引起的有害影响。在这项研究中，我们研究了是否 Aqueous Extract of （AEGl）可减轻酒精引起的行为障碍。雄性（n=30）和雌性 Wistar 大鼠（n=40），72 天大，分别用于行为/生化和口服毒性试验。动物暴露于 5 次 binge（从 35 天大开始）乙醇（3 g/kg/天）或蒸馏水。最后一次 binge 给药后 24 小时，动物接受 AEGl（100 mg/kg/天）或蒸馏水连续 3 天。治疗方案后，进行了旷场、高架十字迷宫、强迫游泳和步下抑制性回避测试。测量氧化应激参数以评估 REDOX 平衡。我们的结果表明，AEGl 可恢复自发水平探索能力、焦虑和抑郁样特征以及 binge 乙醇暴露引起的短期记忆损伤。提取物的行为作用与动物 REDOX 平衡的再平衡有关。因此，AEGl，一种药用蘑菇，可改善由 binge drinking 范式引起的运动、认知和精神病样障碍模型中的行为改变，并作为一种有用的工具，作为酒精源性疾病管理中的食品补充剂。
